製品の概要

  • 製品名Anti-eIF4B (phospho S422) antibody
    eIF4B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to eIF4B (phospho S422)
  • 特異性Detects endogenous levels of eIF4B only when phosphorylated at serine 422.
  • アプリケーション適用あり: ELISA, IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human eIF4B around the phosphorylation site of serine 422 (T-G-SP-E-S).

  • ポジティブ・コントロール
    • Human colon carcinoma tissue, extracts from NIH/3T3cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
ICC/IF Use a concentration of 1 - 5 µg/ml.

ターゲット情報

Anti-eIF4B (phospho S422) antibody 画像

  • All lanes : Anti-eIF4B (phospho S422) antibody (ab59300) at 1/500 dilution

    Lane 1 : extracts from NIH/3T3cells
    Lane 2 : extracts from NIH/3T3cells with immunizing peptide


    Predicted band size : 69 kDa
    Observed band size : 69 kDa
  • Ab59300 at 1/50 dilution staining human colon carcinoma without (left) and with (right) immunizing peptide; paraffin embedded.
  • ICC/IF image of ab59300 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59300, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-eIF4B (phospho S422) antibody (ab59300) 使用論文

This product has been referenced in:
  • Fromm-Dornieden C  et al. Novel polysome messages and changes in translational activity appear after induction of adipogenesis in 3T3-L1 cells. BMC Mol Biol 13:9 (2012). WB . Read more (PubMed: 22436005) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab59300.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"