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Synthetic peptide: PTSGGGKVAP AQPSEEGPGR KDENKVDGMN APKGQTGNSS RGPGDGGNRD, corresponding to a region within C terminal amino acids 505-554 of Human eIF4B
Our Abpromise guarantee covers the use of ab66780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 69 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling eIF4B with ab66780 at 4-8µg/ml. Arrows indicate positively labelled myocardial cells. Magnification: 400X.
ab66780 has not yet been referenced specifically in any publications.