製品の概要

  • 製品名Anti-eIF4A3 antibody
    eIF4A3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to eIF4A3
  • アプリケーション適用あり: IHC-P, IPmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 1-50 of Human eIF4A3 (NP_055555.1).

  • ポジティブ・コントロール
    • Hela whole cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab115022 was affinity purified using an epitope specific to eIF4A3 immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab115022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能ATP-dependent RNA helicase. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed.
    • 組織特異性Ubiquitously expressed.
    • 配列類似性Belongs to the DEAD box helicase family. eIF4A subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • 細胞内局在Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Detected in dendritic layer as well as the nuclear and cytoplasmic (somatic) compartments of neurons. Colocalizes with STAU1 and FMR1 in dendrites.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ATP-dependent RNA helicase DDX48 antibody
      • ATP-dependent RNA helicase eIF4A-3 antibody
      • DDX48 antibody
      • DEAD box protein 48 antibody
      • eIF-4A-III antibody
      • eIF4A-III antibody
      • EIF4A3 antibody
      • eIF4AIII antibody
      • Eukaryotic initiation factor 4A-III antibody
      • Eukaryotic initiation factor 4A-like NUK-34 antibody
      • Eukaryotic translation initiation factor 4A isoform 3 antibody
      • hNMP 265 antibody
      • IF4A3_HUMAN antibody
      • NMP 265 antibody
      • NMP265 antibody
      • Nuclear matrix protein 265 antibody
      • NUK34 antibody
      see all

    Anti-eIF4A3 antibody 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal carcinoma tissue labelling eIF4A3 with ab115022 at 1/1000 (1µg/ml). Detection: DAB.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling eIF4A3 with ab155022 at 1/5000 (0.2µg/ml). Detection: DAB.
    • Detection of eIF4A3 by Western Blot of Immunprecipitate.
      Anti-eIF4A3, at 0.4 µg/ml, staining eIF4A3 Lin Hea whole cell lysate immunoprecipitated using ab115022 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 30 seconds.

    Anti-eIF4A3 antibody (ab115022) 使用論文

    ab115022 has not yet been referenced specifically in any publications.

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