製品の概要

  • 製品名
    Anti-eIF4A3 antibody [2256C1]
    eIF4A3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2256C1] to eIF4A3
  • アプリケーション
    適用あり: WB, Dot blotmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant human DDX48

  • ポジティブ・コントロール
    • Recombinant human DDX48

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM KH2PO4, 8.0mM Na2HPO4, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    ab50741 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • ポリ/モノ
    モノクローナル
  • クローン名
    2256C1
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab50741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Predicted molecular weight: 47 kDa.
Dot blot Use at an assay dependent dilution.

ターゲット情報

  • 機能
    ATP-dependent RNA helicase. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the DEAD box helicase family. eIF4A subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 細胞内局在
    Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Detected in dendritic layer as well as the nuclear and cytoplasmic (somatic) compartments of neurons. Colocalizes with STAU1 and FMR1 in dendrites.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATP-dependent RNA helicase DDX48 antibody
    • ATP-dependent RNA helicase eIF4A-3 antibody
    • DDX48 antibody
    • DEAD box protein 48 antibody
    • eIF-4A-III antibody
    • eIF4A-III antibody
    • EIF4A3 antibody
    • eIF4AIII antibody
    • Eukaryotic initiation factor 4A-III antibody
    • Eukaryotic initiation factor 4A-like NUK-34 antibody
    • Eukaryotic translation initiation factor 4A isoform 3 antibody
    • hNMP 265 antibody
    • IF4A3_HUMAN antibody
    • NMP 265 antibody
    • NMP265 antibody
    • Nuclear matrix protein 265 antibody
    • NUK34 antibody
    see all

Anti-eIF4A3 antibody [2256C1] 画像

プロトコール

Anti-eIF4A3 antibody [2256C1] (ab50741) 使用論文

This product has been referenced in:
  • Haremaki T  et al. Regulation of vertebrate embryogenesis by the exon junction complex core component Eif4a3. Dev Dyn 239:1977-87 (2010). Read more (PubMed: 20549732) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Cardiomyocytes soluble cell extracts)
Loading amount
15 µg
Specification
Cardiomyocytes soluble cell extracts
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 20 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Dec 13 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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