製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to EIF3S2
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rabbit, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human EIF3S2.

    (Peptide available as ab41640.)

  • ポジティブ・コントロール
    • ab41641 gave a positive signal in the following human whole cell lysates: HeLa, Jurkat, A431, HEK293, HepG2, MCF-7 and SHSY-5Y.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab41641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).

ターゲット情報

  • 機能
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • 配列類似性
    Belongs to the eIF-3 subunit I family.
    Contains 5 WD repeats.
  • 翻訳後修飾
    Phosphorylated by TGF-beta type II receptor.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • eIF-3-beta antibody
    • eIF3 beta antibody
    • eIF3 p36 antibody
    • eIF3-beta antibody
    • eIF3-p36 antibody
    • eIF3i antibody
    • EIF3I_HUMAN antibody
    • EIF3S2 antibody
    • EIF3S2, formerly antibody
    • Eukaryotic translation initiation factor 3 subunit 2 antibody
    • Eukaryotic translation initiation factor 3 subunit 2 beta antibody
    • Eukaryotic translation initiation factor 3 subunit I antibody
    • eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD antibody
    • eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa antibody
    • eukaryotic translation initiation factor 3, subunit 2, formerly antibody
    • eukaryotic translation initiation factor 3, subunit I antibody
    • Predicted protein of HQ2242 antibody
    • PRO2242 antibody
    • TGF beta receptor interacting protein 1 antibody
    • TGF-beta receptor-interacting protein 1 antibody
    • TGFbeta receptor interacting protein 1 antibody
    • TGFbeta receptor-interacting protein 1 antibody
    • TRIP 1 antibody
    • TRIP-1 antibody
    • TRIP1 antibody
    see all

画像

  • All lanes : Anti-EIF3S2 antibody (ab41641) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab41641 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab41641, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

参考文献

ab41641 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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