製品の概要

  • 製品名Anti-eIF3K antibody
    eIF3K 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to eIF3K
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide designed within residues: SQLKVWMSKY GWSADESGQI FICSQEE SIKPKNIVE KIDFDSVSSI MASSQ, corresponding to C terminal amino acids 168-218 of Human eIF3K (NP_037366.1)

  • ポジティブ・コントロール
    • Whole cell lysates from HeLa or 293T cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab85968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 25 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • 組織特異性Ubiquitous, with the highest levels of expression in brain, testis and kidney.
  • 配列類似性Belongs to the eIF-3 subunit K family.
  • 細胞内局在Nucleus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARG134 antibody
    • eIF 3 p25 antibody
    • eIF 3 p28 antibody
    • eIF-3 p25 antibody
    • eIF-3 p28 antibody
    • EIF3-p28 antibody
    • eif3k antibody
    • EIF3K_HUMAN antibody
    • EIF3S12 antibody
    • Eukaryotic translation initiation factor 3 subunit 12 antibody
    • Eukaryotic translation initiation factor 3 subunit K antibody
    • HSPC029 antibody
    • M9 antibody
    • MSTP001 antibody
    • Muscle specific gene M9 protein antibody
    • Muscle-specific gene M9 protein antibody
    • PLAC 24 antibody
    • PLAC-24 antibody
    • PLAC24 antibody
    • PRO1474 antibody
    • PTD001 antibody
    see all

Anti-eIF3K antibody 画像

  • All lanes : Anti-eIF3K antibody (ab85968) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 25 kDa


    Exposure time : 10 seconds
  • 1 mg of HeLa whole cell lysate was immunoprecipitated using 3µg ab85968. Subsequently, 20% of IP was loaded per lane and probed with ab85968 at 1µg/ml (lane 1) or with a control IgG (lane 2).
    Detection: chemiluminescence with exposure time of 3 seconds.

Anti-eIF3K antibody (ab85968) 使用論文

ab85968 has not yet been referenced specifically in any publications.

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