製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to EIF3F
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide derived from internal sequence of human EIF3F.

  • ポジティブ・コントロール
    • Human brain tissue. Extracts from Jurkat cells and COLO205 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab74568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 38 kDa.
IHC-P 1/50 - 1/100.
ELISA 1/40000.

ターゲット情報

  • 機能
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
    Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling.
  • 配列類似性
    Belongs to the eIF-3 subunit F family.
    Contains 1 MPN (JAB/Mov34) domain.
  • ドメイン
    The MPN domain mediates deubiquitinating activity.
  • 翻訳後修飾
    Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Deubiquitinating enzyme eIF3f antibody
    • eIF 3 epsilon antibody
    • eIF-3-epsilon antibody
    • eIF3 p47 antibody
    • eIF3 p47 subunit antibody
    • eIF3-epsilon antibody
    • eIF3-p47 antibody
    • eIF3f antibody
    • EIF3F_HUMAN antibody
    • EIF3S5 antibody
    • EIF3S5, formerly antibody
    • Eukaryotic translation initiation factor 3 subunit 5 antibody
    • Eukaryotic translation initiation factor 3 subunit 5, formerly antibody
    • Eukaryotic translation initiation factor 3 subunit F antibody
    • eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD) antibody
    • eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa antibody
    • eukaryotic translation initiation factor 3, subunit F antibody
    • translation initiation factor 3 47 kda subunit antibody
    see all

画像

  • All lanes : Anti-EIF3F antibody (ab74568) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from COLO205 cells
    Lane 3 : Extracts from COLO205 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 38 kDa
    Observed band size : 38 kDa
  • Immunohistochemistry analysis of EIF3F in paraffin-embedded human brain tissue using ab74568, at 1/50-1/100 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

参考文献

This product has been referenced in:
  • Villa N  et al. Human eukaryotic initiation factor 4G (eIF4G) protein binds to eIF3c, -d, and -e to promote mRNA recruitment to the ribosome. J Biol Chem 288:32932-40 (2013). WB ; Human . Read more (PubMed: 24092755) »

See 1 Publication for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab74568.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録