Anti-eIF3e 抗体 [EPR6877] (ab125033)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6877] to eIF3e
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-eIF3e antibody [EPR6877]
eIF3e 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6877] to eIF3e -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide within Human eIF3e aa 200-300. The exact sequence is proprietary.
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ポジティブ・コントロール
- Jurkat, 293T, BxPC-3, A431, Saos 2, HepG2 and NIH 3T3 cell lysates
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 9.40 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6877 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab125033の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 52 kDa).
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 52 kDa). |
ターゲット情報
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機能
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins. -
組織特異性
Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus. -
配列類似性
Belongs to the eIF-3 subunit E family.
Contains 1 PCI domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. Nucleus > PML body. - Information by UniProt
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参照データベース
- Entrez Gene: 3646 Human
- Entrez Gene: 16341 Mouse
- Entrez Gene: 299872 Rat
- Omim: 602210 Human
- SwissProt: P60228 Human
- SwissProt: P60229 Mouse
- SwissProt: Q641X8 Rat
- Unigene: 405590 Human
see all -
別名
- eIF-3 p48 antibody
- eIF3e antibody
- EIF3E_HUMAN antibody
see all
画像
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All lanes : Anti-eIF3e antibody [EPR6877] (ab125033) at 1/1000 dilution
Lanes 1 & 6 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : BxPC3 cell lysate
Lane 4 : A431 cell lysate
Lane 5 : Saos 2 cell lysate
Lane 7 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab125033 は 1 報の論文で使用されています。
- Hoedt E et al. SILAC-based proteomic profiling of the human MDA-MB-231 metastatic breast cancer cell line in response to the two antitumoral lactoferrin isoforms: the secreted lactoferrin and the intracellular delta-lactoferrin. PLoS One 9:e104563 (2014). WB ; Human . PubMed: 25116916