製品の概要
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製品名Anti-EIF2S2 antibody
EIF2S2 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to EIF2S2
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由来種Rabbit
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アプリケーション適用あり: WB, IP, IHC-Pmore details
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種交差性交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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免疫原
Synthetic peptide corresponding to a region between residues 283 and 333 of Human EIF2S2 (NP_003899.2).
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ポジティブ･コントロール
- HeLa, 293T and NIH3T3 whole cell lysates.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab86105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 38 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
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配列類似性Belongs to the eIF-2-beta/eIF-5 family.
- Information by UniProt
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参照データベース
- Entrez Gene: 8894 Human
- Entrez Gene: 67204 Mouse
- Entrez Gene: 296302 Rat
- Omim: 603908 Human
- SwissProt: P20042 Human
- SwissProt: Q99L45 Mouse
- SwissProt: P41035 Rabbit
- Unigene: 429180 Human
see all -
別名
- DKFZp686L18198 antibody
- eIF 2 beta antibody
- eIF-2-beta antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S2 antibody (ab86105)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling EIF2S2 with ab86105 at 1/1000 (1µg/ml). Detection: DAB. -
Western blot - Anti-EIF2S2 antibody (ab86105)All lanes : Anti-EIF2S2 antibody (ab86105) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 38 kDa
Observed band size: 49 kDa (why is the actual band size different from the predicted?)
Exposure time: 10 seconds -
Immunoprecipitation - Anti-EIF2S2 antibody (ab86105)Detection of EIF2S2 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86105 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with exposure time of 10 seconds.
参考文献
ab86105 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"