製品の概要

  • 製品名Anti-EIF2S1 antibody [5E10]
    EIF2S1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5E10] to EIF2S1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-315 of Human EIF2S1, purified from E. coli

  • ポジティブ・コントロール
    • HeLa extract
  • 特記事項Fusion partner: mouse F0 myeloma cells

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 36 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
  • 配列類似性Belongs to the eIF-2-alpha family.
    Contains 1 S1 motif domain.
  • 翻訳後修飾Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation.
  • 細胞内局在Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity).
  • Information by UniProt
  • 参照データベース
  • 別名
    • EIF 2 alpha antibody
    • EIF 2 antibody
    • EIF 2A antibody
    • EIF 2alpha antibody
    • eIF-2-alpha antibody
    • eIF-2A antibody
    • EIF-2alpha antibody
    • EIF2 alpha antibody
    • EIF2 antibody
    • EIF2A antibody
    • EIF2S1 antibody
    • Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody
    • Eukaryotic translation initiation factor 2 subunit 1 alpha antibody
    • Eukaryotic translation initiation factor 2 subunit 1 antibody
    • Eukaryotic translation initiation factor 2 subunit alpha antibody
    • IF2A_HUMAN antibody
    see all

Anti-EIF2S1 antibody [5E10] 画像

  • Anti-EIF2S1 antibody [5E10] (ab119495) at 1/1000 dilution + HeLa extract at 50 µg

    Secondary
    goat anti-mouse HRP
    Developed using the ECL technique

    Predicted band size : 36 kDa
    Observed band size : 36 kDa

Anti-EIF2S1 antibody [5E10] (ab119495) 使用論文

ab119495 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab119495.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"