製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to EIF2D
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 350 and 400 of Human EIF2D (NP_008824.2).

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab108218 was affinity purified using an epitope specific to EIF2D immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab108218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 65 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能
    Trafficking receptor for phosphoglycoproteins. Localizes phosphoglycoproteins within endosomes and at the cell periphery where they participate in specific metabolic processes as well as intercellular adhesion.
  • 配列類似性
    Belongs to the ligatin family.
    Contains 1 PUA domain.
    Contains 1 SUI1 domain.
  • 発生段階
    Found during embryonic development and in early differentiated states.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Eukaryotic translation initiation factor 2D antibody
    • HCA56 antibody
    • Hepatocellular carcinoma associated antigen 56 antibody
    • Hepatocellular carcinoma-associated antigen 56 antibody
    • LGTN antibody
    • LIGA_HUMAN antibody
    • Ligatin antibody
    • OTTHUMP00000034537 antibody
    • OTTHUMP00000034538 antibody
    see all

画像

  • All lanes : Anti-EIF2D antibody (ab108218) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 65 kDa


    Exposure time : 3 minutes
  • ab108218 at 1 µg/ml detecting LGTN in HeLa whole cell lysate by WB following IP.

    Lane 1: IP with an antibody which recognizes an downstream epitope of LGTN
    Lane 2: ab108218 at 6 µg/mg of lysate
    Lane 3: Control IgG.

    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

    Detection: Chemiluminescence with an exposure time of 30 seconds

参考文献

ab108218 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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