Rabbit polyclonal to EIF2D
Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
Synthetic peptide corresponding to a region between residue 350 and 400 of Human EIF2D (NP_008824.2).
HeLa and 293T whole cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab108218 was affinity purified using an epitope specific to EIF2D immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 65 kDa.
Use at 2-5 µg/mg of lysate.
Trafficking receptor for phosphoglycoproteins. Localizes phosphoglycoproteins within endosomes and at the cell periphery where they participate in specific metabolic processes as well as intercellular adhesion.
Belongs to the ligatin family.
Contains 1 PUA domain. Contains 1 SUI1 domain.
Found during embryonic development and in early differentiated states.
Information by UniProt
Eukaryotic translation initiation factor 2D antibody
Hepatocellular carcinoma associated antigen 56 antibody
Western blot - Anti-EIF2D antibody (ab108218)
All lanes : Anti-EIF2D antibody (ab108218) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 65 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-EIF2D antibody (ab108218)
ab108218 at 1 µg/ml detecting LGTN in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an downstream epitope of LGTN
Lane 2: ab108218 at 6 µg/mg of lysate
Lane 3: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds
has not yet been referenced specifically in any publications.
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