Anti-EIF2D 抗体 (ab108218)
Key features and details
- Rabbit polyclonal to EIF2D
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-EIF2D antibody
EIF2D 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EIF2D -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant -
免疫原
Synthetic peptide corresponding to a region between residue 350 and 400 of Human EIF2D (NP_008824.2).
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ポジティブ・コントロール
- HeLa and 293T whole cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab108218 was affinity purified using an epitope specific to EIF2D immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108218の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 65 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 65 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Trafficking receptor for phosphoglycoproteins. Localizes phosphoglycoproteins within endosomes and at the cell periphery where they participate in specific metabolic processes as well as intercellular adhesion. -
配列類似性
Belongs to the ligatin family.
Contains 1 PUA domain.
Contains 1 SUI1 domain. -
発生段階
Found during embryonic development and in early differentiated states. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1939 Human
- Omim: 151625 Human
- SwissProt: P41214 Human
- Unigene: 497581 Human
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別名
- Eukaryotic translation initiation factor 2D antibody
- HCA56 antibody
- Hepatocellular carcinoma associated antigen 56 antibody
see all
画像
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All lanes : Anti-EIF2D antibody (ab108218) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 65 kDa
Exposure time: 3 minutes -
ab108218 at 1 µg/ml detecting LGTN in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an downstream epitope of LGTN
Lane 2: ab108218 at 6 µg/mg of lysate
Lane 3: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab108218 は 2 報の論文で使用されています。
- Sonobe Y et al. A C. elegans model of C9orf72-associated ALS/FTD uncovers a conserved role for eIF2D in RAN translation. Nat Commun 12:6025 (2021). PubMed: 34654821
- Götte B et al. Separate domains of G3BP promote efficient clustering of alphavirus replication complexes and recruitment of the translation initiation machinery. PLoS Pathog 15:e1007842 (2019). PubMed: 31199850