製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to eIF2B4
  • 由来種
    Goat
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-RKDYGSKVSLFSH

    , corresponding to internal sequence amino acids 190-202 of Human eIF2B4 (NP_751945.2; NP_001029288.1; NP_056451.3).

  • ポジティブ・コントロール
    • K562 lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab104941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).

ターゲット情報

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  • Anti-eIF2B4 antibody (ab104941) at 0.3 µg/ml + K562 lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 58 kDa
    Observed band size: 60 kDa (why is the actual band size different from the predicted?)


    Exposure time: 1 hour


    An additional band of unknown identity was also consistently observed at 26 kDa. This band was successfully blocked by incubation with the immunizing peptide.

プロトコール

参考文献

ab104941 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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