1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
機能Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
関連疾患Defects in EIF2B5 are a cause of leukodystrophy with vanishing white matter (VWM) [MIM:603896]. VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy.
配列類似性Belongs to the eIF-2B gamma/epsilon subunits family. Contains 1 W2 domain.
Detection of eIF2B epsilon by Western Blot of Immunprecipitate.
ab91565, at 1 µg/ml, staining eIF2B epsilon in HeLa whole cell lysate immunoprecipitated using ab91565 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
Anti-eIF2B epsilon antibody (ab91565) 使用論文
has not yet been referenced specifically in any publications.