製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to EIF2AK1
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 107-156 (TCSDEFSSLR LHHNRAITHL MRSAKERVRQ DPCEDISRIQ KIRSREVALE) of Human EIF2AK1 (NP_055228).

  • ポジティブ・コントロール
    • Fetal lung lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab84980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions.
  • 組織特異性
    Expressed predominantly in erythroid cells. At much lower levels, expressed in hepatocytes (at protein level).
  • 配列類似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 2 HRM (heme regulatory motif) repeats.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Activated by autophosphorylation; phosphorylated predominantly on serine and threonine residues, but also on tyrosine residues. Autophosphorylation at Thr-488 is required for kinase activation. The active autophosphorylated form apparently is largely refractory to cellular heme fluctuations.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E2AK1_HUMAN antibody
    • EC 2.7.11.1 antibody
    • Eif2ak1 antibody
    • Eukaryotic translation initiation factor 2 alpha kinase 1 antibody
    • Eukaryotic translation initiation factor 2-alpha kinase 1 antibody
    • HCR antibody
    • Heme controlled repressor antibody
    • Heme regulated eukaryotic initiation factor eIF 2 alpha kinase antibody
    • Heme regulated inhibitor antibody
    • Heme regulated initiation factor 2 alpha kinase antibody
    • Heme regulated repressor antibody
    • Heme sensitive initiation factor 2a kinase antibody
    • Heme-controlled repressor antibody
    • Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase antibody
    • Heme-regulated inhibitor antibody
    • Hemin sensitive initiation factor 2 alpha kinase antibody
    • Hemin-sensitive initiation factor 2-alpha kinase antibody
    • HRI antibody
    • KIAA1369 antibody
    • PRO1362 antibody
    see all

Anti-EIF2AK1 antibody 画像

  • ab84980 staining EIF2AK1 in lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab84980 staining EIF2AK1 in brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-EIF2AK1 antibody (ab84980) 使用論文

This product has been referenced in:
  • Taniuchi S  et al. Integrated stress response of vertebrates is regulated by four eIF2a kinases. Sci Rep 6:32886 (2016). WB ; Mouse . Read more (PubMed: 27633668) »

See 1 Publication for this product

Product Wall

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (7.5)
Sample
Human Cell lysate - whole cell (HEK293T cell line)
Specification
HEK293T cell line
Blocking step
Blocking One as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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投稿 Feb 27 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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