Anti-EHMT2/G9A 抗体 - ChIP Grade (ab40542)

製品の概要

  • 製品名Anti-EHMT2/G9A antibody - ChIP Grade
    EHMT2/G9A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EHMT2/G9A - ChIP Grade
  • アプリケーション適用あり: IP, ChIP, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Fusion protein corresponding to Human EHMT2/ G9A aa 831-1001.
    Database link: Q96KQ7

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab40542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/2000 - 1/2500. Predicted molecular weight: 132 kDa. For endogeneous detection (without G9a over expression): use nuclear extracts and block overnight or perform IP prior to WB.

ターゲット情報

  • 機能Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
  • 組織特異性Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.
  • 配列類似性Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 7 ANK repeats.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • ドメインThe SET domain mediates interaction with WIZ.
    The ANK repeats bind H3K9me1 and H3K9me2.
  • 翻訳後修飾Methylated at Lys-185; automethylated.
  • 細胞内局在Nucleus. Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Ankyrin repeat containing protein antibody
    • Bat 8 antibody
    • Bat8 antibody
    • C6orf30 antibody
    • DKFZp686H08213 antibody
    • EHMT 2 antibody
    • Ehmt2 antibody
    • EHMT2_HUMAN antibody
    • Euchromatic histone lysine N methyltransferase 2 antibody
    • Euchromatic histone-lysine N-methyltransferase 2 antibody
    • FLJ35547 antibody
    • G 9a antibody
    • G9 a antibody
    • G9a protein antibody
    • G9A antibody
    • G9A histone methyltransferase antibody
    • GAT8 antibody
    • H3 K9 HMTase 3 antibody
    • H3-K9-HMTase 3 antibody
    • Histone H3 K9 methyltransferase 3 antibody
    • Histone H3 K9 methyltransferase3 antibody
    • Histone H3-K9 methyltransferase 3 antibody
    • Histone lysine N methyltransferase antibody
    • Histone lysine N methyltransferase EHMT2 antibody
    • Histone lysine N methyltransferase, H3 lysine 9 specific 3 antibody
    • Histone lysine N methyltransferase, H3 lysine 9 specific3 antibody
    • Histone-lysine N-methyltransferase EHMT2 antibody
    • HLA B associated transcript 8 antibody
    • HLA-B-associated transcript 8 antibody
    • KMT 1C antibody
    • KMT1 C antibody
    • Lysine N methyltransferase 1C antibody
    • Lysine N-methyltransferase 1C antibody
    • NG 36 antibody
    • NG36 antibody
    • Protein G9a antibody
    see all

Anti-EHMT2/G9A antibody - ChIP Grade 画像

  • All lanes : Anti-EHMT2/G9A antibody - ChIP Grade (ab40542) at 1/2500 dilution

    Lane 1 : Untransfected HeLa cell extract
    Lane 2 : G9a transfected HeLa cell extract


    Predicted band size : 132 kDa
    Observed band size : 132 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • ab40542 (1:2000) staining KMT1C in human prostate using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-EHMT2/G9A antibody - ChIP Grade (ab40542) 使用論文

This product has been referenced in:
  • Rao VK  et al. G9a promotes proliferation and inhibits cell cycle exit during myogenic differentiation. Nucleic Acids Res 44:8129-43 (2016). WB, ChIP ; Mouse . Read more (PubMed: 27229136) »
  • Lei W  et al. Homocysteine Induces Collagen I Expression by Downregulating Histone Methyltransferase G9a. PLoS One 10:e0130421 (2015). WB ; Mouse, Human . Read more (PubMed: 26192994) »

See all 10 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (brain)
Permeabilization Yes - Triton 0.5%
Specification brain
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative Paraformaldehyde
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投稿 Sep 08 2015

Thank you for your reply. I am sorry that this product has not met your needs.Your credit note ID is xxxxx. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing...

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Thank you for contacting Abcam.

I am sorry about that you are experiencing some problems with ab40542 in ChiP. The antibody is covered under our Abpromise for six months and is guaranteed to work in ChIP on human samples . If we cannot resolve...

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Thank you for contacting us.
For ab40542: As immunogen, the recombinant fusion protein, corresponding to amino acids 831-1001 of Human G9a was used to raise the antibody. The epitope has not been mapped.
As for the DNMT1 (DNA methyl transfera...

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Thank you for your reply. I am sorry that this antibody is producing poor results in WB. Based on the images provided, I suspect the antibody has lost specificity with age. It is possible that the results may be improved with troubleshooting, but it wo...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human pancreatic ductal adenocarcinoma)
Specification human pancreatic ductal adenocarcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH=6
Permeabilization Yes - PBS+0.025% TritonX100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Jan 28 2011

Application Western blot
Sample Human Cell lysate - other (A549)
Loading amount 75000 cells
Specification A549
Treatment multiple (DFO & CoCl2)
Gel Running Conditions Non-reduced Denaturing (8-16)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 50000µg/mL · Temperature: 22°C
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Mr. Ted Natoli

Verified customer

投稿 Nov 16 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Rat Tissue lysate - whole (muscle)
Specification muscle
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step Real-time PCR
Positive control Genes that are positive for H3K9me2 and H3K27me2, i.e. in a repressed state of transcription.
Negative control Genes in an active state of transcription, negative for H3K9me2 and H3K27me2.
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投稿 Jul 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (Hela)
Loading amount 60 µg
Specification Hela
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Jan 15 2010

Application Western blot
Sample Human Cell lysate - nuclear (HeLa)
Loading amount 25 µg
Specification HeLa
Treatment G9a specific siRNA
Gel Running Conditions Reduced Denaturing (4-20% gel)
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: 25°C
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投稿 Dec 08 2009

1-10 of 13 Abreviews or Q&A

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