Anti-EHMT1/GLP 抗体 [B0422] - ChIP Grade (ab41969)

製品の概要

  • 製品名
    Anti-EHMT1/GLP antibody [B0422] - ChIP Grade
    EHMT1/GLP 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [B0422] to EHMT1/GLP - ChIP Grade
  • アプリケーション
    適用あり: ChIP, ICC/IF, WB, ELISA, IPmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment corresponding to Mouse EHMT1/GLP aa 134-234.
    Database link: Q5DW34

  • ポジティブ・コントロール
    • WT mouse ES cell

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab41969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent concentration. PubMed: 18066052
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 134 kDa.
ELISA Use a concentration of 0.1 µg/ml.
IP Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.
  • 組織特異性
    Widely expressed.
  • 関連疾患
    Defects in EHMT1 are the cause of chromosome 9q subtelomeric deletion syndrome (9q- syndrome) [MIM:610253]. Common features seen in these patients are severe mental retardation, hypotonia, brachy(micro)cephaly, epileptic seizures, flat face with hypertelorism, synophrys, anteverted nares, cupid bow or tented upper lip, everted lower lip, prognathism, macroglossia, conotruncal heart defects, and behavioral problems.
  • 配列類似性
    Belongs to the histone-lysine methyltransferase family.
    Contains 8 ANK repeats.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • ドメイン
    The ANK repeats recognize and bind RELA subunit of NF-kappa-B, when RELA is monomethylated at 'Lys-310' (By similarity). They also specifically recognize and bind H3K9me1 and H3K9me2.
    The SET domain mediates interaction with WIZ.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus. Chromosome. Associates with euchromatic regions.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bA188C12.1 antibody
    • DKFZp667M072 antibody
    • EHMT 1 antibody
    • EHMT1 antibody
    • EHMT1_HUMAN antibody
    • Eu-HMTase1 antibody
    • Euchromatic histone lysine N methyltransferase 1 antibody
    • Euchromatic histone methyltransferase 1 antibody
    • Euchromatic histone-lysine N-methyltransferase 1 antibody
    • EUHMTASE1 antibody
    • FLJ12879 antibody
    • FP13812 antibody
    • G9a like protein 1 antibody
    • G9a like protein antibody
    • G9a-like protein 1 antibody
    • GLP 1 antibody
    • GLP antibody
    • GLP1 antibody
    • H3 K9 HMTase 5 antibody
    • H3-K9-HMTase 5 antibody
    • Histone H3 K9 methyltransferase 5 antibody
    • Histone H3-K9 methyltransferase 5 antibody
    • Histone lysine N methyltransferase H3 lysine 9 specific 5 antibody
    • Histone-lysine N-methyltransferase EHMT1 antibody
    • KIAA1876 antibody
    • Lysine N methyltransferase 1D antibody
    • Lysine N-methyltransferase 1D antibody
    • RP11 188C12.1 antibody
    see all

画像

  • Ab41969 at 5 µg/ml staining WT mouse ES cell (Positive)
  • Ab41969 at 5µg/ml staining GLP KO mouse ES cell (Negative)

参考文献

This product has been referenced in:

See all 11 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Dermal Microvascular Endothelial Cells)
Permeabilization
Yes - 0.1% Triton-X100
Specification
Human Dermal Microvascular Endothelial Cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Jul 13 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - nuclear (B cells)
Negative control
IgG
Specification
B cells
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 5 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control
Gene Promoter
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投稿 Apr 27 2017

Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (Dermal Endothelial Cells (Primary))
Total protein in input
300 µg
Immuno-precipitation step
Protein A/G
Specification
Dermal Endothelial Cells (Primary)
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投稿 Apr 24 2017

Application
Western blot
Sample
Human Cell lysate - nuclear (Dermal Endothelial cells (Primary))
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
Dermal Endothelial cells (Primary)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Apr 21 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (C2C12 myoblast cells)
Specification
C2C12 myoblast cells
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Verified customer

投稿 May 07 2013

Thank you so much for your reply.
Below are the testing discount codes for both GLP antibodies. At the bottom of the email is the information about the free of charge replacement for ab41969. A PO number is not needed on my end for sending you the...

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Thank you for your reply.
Yes, I think you are understanding this correctly.
I will be able to replace ab41969 free of charge with one vial of either ab63161 or ab84751. I am not able to send you both. Unfortunately, we do not offer sample si...

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Thank you for your reply and the additional information which will be very helpful to us to evaluate the antibody.
I have checked, but unfortunately we do not have another new lot for ab41969 in stock right now. Instead, I can send you a replaceme...

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Thank you for contacting us.
I am very sorry to hear that the antibody ab41969 is giving you problems.
Could you please let me know the following details for this antibody:
1) How were your samples prepared (e.g. lysis buffer)?
2) H...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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