製品の概要

  • 製品名Anti-EHHADH antibody
    EHHADH 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to EHHADH
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein (Human) (NP_001957.2)

  • ポジティブ・コントロール
    • Human liver tissue lysate HeLa cells EHHADH transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 79 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Antibody must be purified (with Protein A) before use.

ターゲット情報

  • 組織特異性Liver and kidney. Lower amounts seen in the brain.
  • パスウェイLipid metabolism; fatty acid beta-oxidation.
  • 配列類似性In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
    In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
  • 翻訳後修飾Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids.
  • 細胞内局在Peroxisome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3 hydroxyacyl CoA dehydrogenase antibody
    • 3,2 trans enoyl CoA isomerase antibody
    • 3-hydroxyacyl-CoA dehydrogenase antibody
    • ECHD antibody
    • ECHP_HUMAN antibody
    • EHHADH antibody
    • Enoyl Coenzyme A, hydratase/3 hydroxyacyl Coenzyme A dehydrogenase antibody
    • L 3 hydroxyacyl CoA dehydrogenase antibody
    • L bifunctional protein, peroxisomal antibody
    • L PBE antibody
    • LBFP antibody
    • LBP antibody
    • MGC120586 antibody
    • MS730 antibody
    • PBE antibody
    • PBFE antibody
    • Peroxisomal bifunctional enzyme antibody
    • Peroxisomal enoyl CoA hydratase antibody
    see all

Anti-EHHADH antibody 画像

  • Anti-EHHADH antibody (ab72795) at 1/500 dilution + Human liver tissue lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 79 kDa
    Observed band size : 79 kDa
  • All lanes : Anti-EHHADH antibody (ab72795) at 1/500 dilution

    Lane 1 : EHHADH transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 79 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of fixed and permeabilised HeLa cells using purified ab72795 at 10µg/ml followed by a fluorescein-conjugated secondary antibody.

Anti-EHHADH antibody (ab72795) 使用論文

This product has been referenced in:

See 1 Publication for this product

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