製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to EHHADH
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 673-723 of Human EHHADH using the numbering given in entry NP_001957.2.

  • ポジティブ・コントロール
    • Whole cell lysate from 293T, Jurkat, HeLa

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab123490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2500. Predicted molecular weight: 79 kDa.
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 組織特異性
    Liver and kidney. Lower amounts seen in the brain.
  • パスウェイ
    Lipid metabolism; fatty acid beta-oxidation.
  • 配列類似性
    In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
    In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
  • 翻訳後修飾
    Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids.
  • 細胞内局在
    Peroxisome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3 hydroxyacyl CoA dehydrogenase antibody
    • 3,2 trans enoyl CoA isomerase antibody
    • 3-hydroxyacyl-CoA dehydrogenase antibody
    • ECHD antibody
    • ECHP_HUMAN antibody
    • EHHADH antibody
    • Enoyl Coenzyme A, hydratase/3 hydroxyacyl Coenzyme A dehydrogenase antibody
    • L 3 hydroxyacyl CoA dehydrogenase antibody
    • L bifunctional protein, peroxisomal antibody
    • L PBE antibody
    • LBFP antibody
    • LBP antibody
    • MGC120586 antibody
    • MS730 antibody
    • PBE antibody
    • PBFE antibody
    • Peroxisomal bifunctional enzyme antibody
    • Peroxisomal enoyl CoA hydratase antibody
    see all

画像

  • All lanes : Anti-EHHADH antibody (ab123490) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 79 kDa


    Exposure time : 3 minutes
  • Lane 1: Immunoprecipitation from 293T whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an upstream epitope of EHHADH.
    Lane 2: Detection of Human EHHADH by Immunoprecipitation from 293T whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123490 at 6 µg/mg lysate for IP.
    Lane 3: Immunoprecipitation from 293T whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
    Subsequent Western blot detection used ab123490 at 1 µg/ml.
    Detection: Chemiluminescence with an exposure time of 3 minutes.

参考文献

ab123490 has not yet been referenced specifically in any publications.

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