Anti-EHHADH 抗体 (ab123490)
Key features and details
- Rabbit polyclonal to EHHADH
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-EHHADH antibody
EHHADH 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EHHADH -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla, Orangutan -
免疫原
Synthetic peptide, corresponding to a region within amino acids 673-723 of Human EHHADH using the numbering given in entry NP_001957.2.
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ポジティブ・コントロール
- Whole cell lysate from 293T, Jurkat, HeLa
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab123490の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/2500. Predicted molecular weight: 79 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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WB
1/500 - 1/2500. Predicted molecular weight: 79 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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組織特異性
Liver and kidney. Lower amounts seen in the brain. -
パスウェイ
Lipid metabolism; fatty acid beta-oxidation. -
配列類似性
In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family. -
翻訳後修飾
Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids. -
細胞内局在
Peroxisome. - Information by UniProt
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参照データベース
- Entrez Gene: 1962 Human
- Omim: 607037 Human
- SwissProt: Q08426 Human
- Unigene: 429879 Human
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別名
- 3 hydroxyacyl CoA dehydrogenase antibody
- 3,2 trans enoyl CoA isomerase antibody
- 3-hydroxyacyl-CoA dehydrogenase antibody
see all
画像
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All lanes : Anti-EHHADH antibody (ab123490) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 79 kDa
Exposure time: 3 minutes -
Lane 1: Immunoprecipitation from 293T whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an upstream epitope of EHHADH.
Lane 2: Detection of Human EHHADH by Immunoprecipitation from 293T whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123490 at 6 µg/mg lysate for IP.
Lane 3: Immunoprecipitation from 293T whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab123490 at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab123490 は 1 報の論文で使用されています。
- Zhao C et al. The Renoprotective Effects of Naringenin (NGN) in Gestational Pregnancy. Diabetes Metab Syndr Obes 13:53-63 (2020). PubMed: 32021351