製品の概要

  • 製品名
    Anti-EHHADH antibody [7F6AF11]
    EHHADH 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [7F6AF11] to EHHADH
  • アプリケーション
    適用あり: IP, ICCmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human liver mitochondria.

  • ポジティブ・コントロール
    • Human liver mitochondria and HpG2 cells.
  • 特記事項

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent dilution.
ICC Use a concentration of 1 µg/ml.

ターゲット情報

  • 組織特異性
    Liver and kidney. Lower amounts seen in the brain.
  • パスウェイ
    Lipid metabolism; fatty acid beta-oxidation.
  • 配列類似性
    In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
    In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
  • 翻訳後修飾
    Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids.
  • 細胞内局在
    Peroxisome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3 hydroxyacyl CoA dehydrogenase antibody
    • 3,2 trans enoyl CoA isomerase antibody
    • 3-hydroxyacyl-CoA dehydrogenase antibody
    • ECHD antibody
    • ECHP_HUMAN antibody
    • EHHADH antibody
    • Enoyl Coenzyme A, hydratase/3 hydroxyacyl Coenzyme A dehydrogenase antibody
    • L 3 hydroxyacyl CoA dehydrogenase antibody
    • L bifunctional protein, peroxisomal antibody
    • L PBE antibody
    • LBFP antibody
    • LBP antibody
    • MGC120586 antibody
    • MS730 antibody
    • PBE antibody
    • PBFE antibody
    • Peroxisomal bifunctional enzyme antibody
    • Peroxisomal enoyl CoA hydratase antibody
    see all

画像

  • EHHADH immunocaptured from Human liver mitochondria using ab110299.
  • Immunocytochemistry image of EHHADH stained Human HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated ab110299 at 1µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in peroxisome.

参考文献

ab110299 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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