Anti-EHHADH 抗体 [7F6AF11] (ab110299)
Key features and details
- Mouse monoclonal [7F6AF11] to EHHADH
- Suitable for: IP, ICC
- Reacts with: Human
- Isotype: IgG3
製品の概要
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製品名
Anti-EHHADH antibody [7F6AF11]
EHHADH 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7F6AF11] to EHHADH -
由来種
Mouse -
アプリケーション
適用あり: IP, ICCmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is considered to be commercially sensitive.
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ポジティブ・コントロール
- ICC: HepG2 cells. IP: Enriched subcellular organelles from human liver.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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精製度
Proprietary Purification -
特記事項(精製)
Near homogeneity as judged by SDS-PAGE (>95% purity). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
ポリ/モノ
モノクローナル -
クローン名
7F6AF11 -
アイソタイプ
IgG3 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110299の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent dilution.
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ICC |
Use a concentration of 1 µg/ml.
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特記事項 |
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IP
Use at an assay dependent dilution. |
ICC
Use a concentration of 1 µg/ml. |
ターゲット情報
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組織特異性
Liver and kidney. Lower amounts seen in the brain. -
パスウェイ
Lipid metabolism; fatty acid beta-oxidation. -
配列類似性
In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family. -
翻訳後修飾
Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids. -
細胞内局在
Peroxisome. - Information by UniProt
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参照データベース
- Entrez Gene: 1962 Human
- Omim: 607037 Human
- SwissProt: Q08426 Human
- Unigene: 429879 Human
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別名
- 3 hydroxyacyl CoA dehydrogenase antibody
- 3,2 trans enoyl CoA isomerase antibody
- 3-hydroxyacyl-CoA dehydrogenase antibody
see all
画像
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Immonprecipitation analysis of enriched subcellular organelles from human liver, which contains mitochondria and peroxisome. EHHADH immunocaptured using ab110299.
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Immunocytochemistry image of EHHADH stained Human HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated ab110299 at 1µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in peroxisome.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab110299 は論文での使用が確認できていません。