製品の概要

  • 製品名Anti-EHF antibody
    EHF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EHF
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee
  • 免疫原

    A region within synthetic peptide: PFQEFDINGE HLCSMSLQEF TRAAGTAGQL LYSNLQHLKW NGQCSSDLFQ, corresponding to N terminal amino acids 74-123 of Human EHF

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab24337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Transcriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. May act as a repressor for a specific subset of ETS/AP-1-responsive genes and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. Binds to DNA sequences containing the consensus nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter. May contribute to development and carcinogenesis by acting as a tumor suppressor gene or anti-oncogene.
  • 組織特異性Expressed exclusively in tissues with a high content of epithelial cells. Highly expressed in salivary gland, mammary gland, prostate, and lung. Weakly expressed in kidney and colon. Not detected in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, testis, ovary, small intestine or peripheral blood leukocytes.
  • 配列類似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • ドメインThe PNT domain acts as a transcriptional activator.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EHF antibody
    • EHF_HUMAN antibody
    • Epithelium-specific Ets transcription factor 3 antibody
    • ESE-3 antibody
    • ESE3 antibody
    • ESE3 transcription factor antibody
    • ESEJ antibody
    • Ets domain transcription factor antibody
    • ETS domain-containing transcription factor antibody
    • ETS homologous factor antibody
    • hEHF antibody
    see all

Anti-EHF antibody 画像

  • Anti-EHF antibody (ab24337) at 1.25 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000-1/100000

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
    ab24337 was used at a dilution of 1.25 ug/ml in 5% skim milk / PBS buffer, with a secondary HRP conjugated anti-Rabbit IgG. Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling EHF with ab24337 at 8µg/ml. Renal tubule epithelial cells labelled are indicated with arrows. Magnification: X400.

Anti-EHF antibody (ab24337) 使用論文

ab24337 has not yet been referenced specifically in any publications.

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