製品の概要

  • 製品名Anti-EGR4 antibody
    EGR4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EGR4
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide (Human)

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab50636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Peptide ELISA titre: 1/312500.

WB Use a concentration of 0.0625 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 51 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性EGR4(Early Growth Response protein 4) is a zinc-finger transcription factor that activates the transcription of genes whose products are required for mitogenesis and differentiation. EGR4 is closely related to nerve growth factor-induced clone A (NGFI-A or EGR1), EGR2, and EGR3. These four early response (immediate early) proteins all contain very similar zinc-finger DNA binding domains and five highly homologous subdomains. EGR4 functionally cooperate with NFAT proteins and induce expression of IL2 and TNFalpha. Early growth response proteins (EGR) and nuclear factors of activated T cells (NFAT) form heterodimers and regulate proinflammatory cytokine gene expression.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • AT133 antibody
    • Early growth response protein 4 antibody
    • Nerve growth factor induced clone C antibody
    • NGFI C antibody
    • NGFIC antibody
    • PAT133 antibody
    see all

Anti-EGR4 antibody 画像

  • Anti-EGR4 antibody (ab50636) at 0.0625 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution.

    Predicted band size : 51 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)

Anti-EGR4 antibody (ab50636) 使用論文

ab50636 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"