リコンビナント
RabMAb

Anti-EGR2 抗体 [EPR4004] (ab108399)

製品の概要

  • 製品名
    Anti-EGR2 antibody [EPR4004]
    EGR2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4004] to EGR2
  • アプリケーション
    適用あり: WBmore details
    適用なし: ICC,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human EGR2.

  • ポジティブ・コントロール
    • LnCaP, HepG2, MCF7, and SH SY5Y cell lysates.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
  • 追加情報
    Is unsuitable for ICC,IHC-P or IP.
  • ターゲット情報

    • 機能
      Sequence-specific DNA-binding transcription factor. Binds to two specific DNA sites located in the promoter region of HOXA4.
    • 関連疾患
      Defects in EGR2 are a cause of congenital hypomyelination neuropathy (CHN) [MIM:605253]. Inheritance can be autosomal dominant or recessive. Recessive CHN is also known as Charcot-Marie-Tooth disease type 4E (CMT4E). CHN is characterized clinically by early onset of hypotonia, areflexia, distal muscle weakness, and very slow nerve conduction velocities.
      Defects in EGR2 are a cause of Charcot-Marie-Tooth disease type 1D (CMT1D) [MIM:607678]. CMT1D is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet.
      Defects in EGR2 are a cause of Dejerine-Sottas syndrome (DSS) [MIM:145900]; also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie-Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine-Sottas syndrome.
    • 配列類似性
      Belongs to the EGR C2H2-type zinc-finger protein family.
      Contains 3 C2H2-type zinc fingers.
    • 翻訳後修飾
      Ubiquitinated by WWP2 leading to proteasomal degradation.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AT591 antibody
      • CMT1D antibody
      • CMT4E antibody
      • DKFZp686J1957 antibody
      • E3 SUMO-protein ligase EGR2 antibody
      • Early growth response 2 antibody
      • Early growth response protein 2 antibody
      • EGR-2 antibody
      • egr2 antibody
      • EGR2_HUMAN antibody
      • FLJ14547 antibody
      • KROX 20 Drosophila homolog antibody
      • Krox 20 homolog Drosophila antibody
      • KROX-20, Drosophila, homolog (early growth response-2) antibody
      • KROX20 antibody
      • Krox20 protein antibody
      • Zinc finger protein Krox-20 antibody
      see all

    画像

    • All lanes : Anti-EGR2 antibody [EPR4004] (ab108399) at 1/1000 dilution

      Lane 1 : LnCaP cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : SH SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 53 kDa

    参考文献

    This product has been referenced in:
    • Collombet S  et al. Logical modeling of lymphoid and myeloid cell specification and transdifferentiation. Proc Natl Acad Sci U S A 114:5792-5799 (2017). WB . Read more (PubMed: 28584084) »
    • Zhuo W  et al. MicroRNA-20a promotes the proliferation and cell cycle of human osteosarcoma cells by suppressing early growth response 2 expression. Mol Med Rep 12:4989-94 (2015). WB . Read more (PubMed: 26238942) »

    See all 3 Publications for this product

    レビューと Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - Triton X-100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

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    Verified customer

    投稿 Jul 06 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (brain)
    Gel Running Conditions
    Reduced Denaturing (Bis-Tris 4-12%)
    Loading amount
    30 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Abcam user community

    Verified customer

    投稿 Jun 03 2016

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Specification
    U2OS cells
    Treatment
    50 pM TGF-beta time course
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

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    Verified customer

    投稿 Sep 03 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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