Anti-Egr1 抗体 [mAbcam55160] (ab55160)
製品の概要
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製品名Anti-Egr1 antibody [mAbcam55160]
Egr1 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [mAbcam55160] to Egr1
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由来種Mouse
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アプリケーション適用あり: WB, IP, IHC-P, Flow Cytmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human Egr1.
(Peptide available as ab56196.)
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ポジティブ･コントロール
- This antibody gave a positive signal in the following lysates: HeLa Whole Cell Lysate; HeLa Nuclear lysate - TPA stimulated 2 hr
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特記事項
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度IgG fraction
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ポリ/モノモノクローナル
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クローン名mAbcam55160
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アイソタイプIgG2a
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研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab55160 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 5 µg/ml. Detects a band of approximately 55, 85 kDa (predicted molecular weight: 58 kDa). | |
| IP | Use a concentration of 5 µg/ml. | |
| IHC-P | 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | Use 1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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機能Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
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配列類似性Belongs to the EGR C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 407125 Cow
- Entrez Gene: 1958 Human
- Entrez Gene: 13653 Mouse
- Entrez Gene: 24330 Rat
- Omim: 128990 Human
- SwissProt: Q29W20 Cow
- SwissProt: P18146 Human
- SwissProt: P08046 Mouse
see all -
別名
- AT225 antibody
- Early growth response 1 antibody
- Early growth response protein 1 antibody
see all
画像
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![Immunoprecipitation - Anti-Egr1 antibody [mAbcam55160] (ab55160)](http://a.abcam.com/ps/products/55/ab55160/Images/ab55160-195054-IPV020ab5516020mMod.jpg)
Immunoprecipitation - Anti-Egr1 antibody [mAbcam55160] (ab55160)Egr1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Egr1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab55160.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 55kDa; Egr1
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![Western blot - Egr1 antibody [mAbcam55160] (ab55160)](http://a.abcam.com/ps/datasheet/Images/55/ab55160/ab55160_2.jpg)
Western blot - Egr1 antibody [mAbcam55160] (ab55160)All lanes : Anti-Egr1 antibody [mAbcam55160] (ab55160) at 5 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :HeLa nuclear extract lysate (TPA stimulated 2 hr) (ab14653)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 55,85 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Egr1 antibody [mAbcam55160] (ab55160)](http://a.abcam.com/ps/datasheet/images/55/ab55160/Egr1-Primary-antibodies-ab55160-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Egr1 antibody [mAbcam55160] (ab55160)This image is courtesy of an abreview submitted by Antibody Solutions Ltd.ab55160 (1/250) staining Egr1 in paraffin-embedded Human placenta tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. The secondary antibody was goat anti rabbit/mouse conjugated to HRP. For further experimental details please refer to abreview.
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](http://a.abcam.com/ps/datasheet/images/55/ab55160/Egr1-Primary-antibodies-ab55160-10.jpg)
Flow Cytometry-Anti-Egr1 antibody [mAbcam55160](ab55160)Overlay histogram showing HeLa cells stained with ab55160 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55160, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
参考文献
This product has been referenced in:
- Zhang YM et al. Autophagy-related gene LRRK2 is likely a susceptibility gene for systemic lupus erythematosus in northern Han Chinese. Oncotarget 8:13754-13761 (2017). Read more (PubMed: 28099919) »
- Huang Q et al. HTLV-1 Tax upregulates early growth response protein 1 through nuclear factor-?B signaling. Oncotarget 8:51123-51133 (2017). Read more (PubMed: 28881635) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"