Anti-EGFR (phospho Y1173) 抗体 [E124] (ab32578)


  • 製品名Anti-EGFR (phospho Y1173) antibody [E124]
    EGFR 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E124] to EGFR (phospho Y1173)
  • 特異性The antibody detects EGFR phosphorylated on Tyrosine 1173 of the mature human isoform 1 (corresponding to Y1197 from the precursor)
  • アプリケーション適用あり: ICC/IF, WB, IHC-P, IPmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic phospho-peptide corresponding to residues surrounding Tyr1173 of human EGF receptor.

  • ポジティブ・コントロール
    • WB: EGF-treated A431 cells lysate IHC: Phosphatase-treated, paraffin-embedded skin carcinoma
  • 特記事項

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab32578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/25 - 1/50.
WB 1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 170 kDa).
IHC-P 1/25 - 1/50.
IP 1/10.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
      Isoform 2 may act as an antagonist of EGF action.
    • 組織特異性Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
    • 関連疾患Lung cancer
      Inflammatory skin and bowel disease, neonatal, 2
    • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
      Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
      Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
    • 細胞内局在Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
    • Information by UniProt
    • 参照データベース
    • 別名
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    Anti-EGFR (phospho Y1173) antibody [E124] 画像

    • ab32578 (at 1/50) staining (A) untreated and (B) Phosphatase-treated skin carcinoma tissue by IHC-P.
    • All lanes : Anti-EGFR (phospho Y1173) antibody [E124] (ab32578) at 1/1000 dilution

      Lane 1 : A431
      Lane 2 : A431 + EGF cell lysates

      Predicted band size : 170 kDa
    • Immunofluorescent staining of untreated A431 cells using ab32578.
    • Immunofluorescent staining of Phosphatase-treated A431 cells using ab32578.

    Anti-EGFR (phospho Y1173) antibody [E124] (ab32578) 使用論文

    This product has been referenced in:
    • Chang YL  et al. Regulation of estrogen receptor a function in oral squamous cell carcinoma cells by FAK signaling. Endocr Relat Cancer 21:555-65 (2014). Human . Read more (PubMed: 24825747) »
    • Gerdes CA  et al. GA201 (RG7160): a novel, humanized, glycoengineered anti-EGFR antibody with enhanced ADCC and superior in vivo efficacy compared with cetuximab. Clin Cancer Res 19:1126-38 (2013). Read more (PubMed: 23209031) »

    See all 9 Publications for this product

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    This antibody is not a purified product. However the IgG concentration in the supernatant refers to the specific IgG.