Anti-EGFR (phospho Y1092) 抗体 [EP774Y] (ab40815)

製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab40815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration. PubMed: 18948956
WB 1/500 - 1/5000. Predicted molecular weight: 170 kDa.Can be blocked with EGFR (phospho Y1092) peptide (ab190788).
ICC/IF 1/250 - 1/500.
IHC-P 1/250 - 1/500.

ターゲット情報

  • 機能Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.
  • 組織特異性Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
  • 関連疾患Lung cancer
    Inflammatory skin and bowel disease, neonatal, 2
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
    Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
  • 細胞内局在Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
  • Information by UniProt
  • 参照データベース
  • 別名
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • ERBB antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Anti-EGFR (phospho Y1092) antibody [EP774Y] 画像

  • Immunohistochemical staining of untreated (A) and Phosphatase- treated (B) paraffin-embedded breast adenocarcinoma tissue using ab40815.
  • All lanes : Anti-EGFR (phospho Y1092) antibody [EP774Y] (ab40815) at 1/5000 dilution

    Lane 1 : Untreated A431 (Human epidermoid carcinoma cell line) whole cell lysates
    Lane 2 : A431 (Human epidermoid carcinoma cell line) treated with 100 ng/ml EGF for 10 minutes whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 170 kDa
    Observed band size : 170 kDa


    Exposure time : 1 second

    Blocking/Diluting buffer 5% NFDM/TBST

  • All lanes : Anti-EGFR (phospho Y1092) antibody [EP774Y] (ab40815) at 1/5000 dilution

    Lane 1 : A431 treated with Epidermal Growth Factor (EGF) whole cell lysate
    Lane 2 : Untreated A431 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 170 kDa
    Observed band size : 170 kDa


    Exposure time : 1 minute

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Dot blot analysis of EGFR (pY1092) peptide (Lane 1), SMAD5 (unmodified) peptide labelling EGFR (pY1092) with ab40815 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • ab40815 showing positive staining in Papillary carcinoma of thyroid gland tissue.

  • ab40815 showing positive staining in Glioma tissue.

  • ab40815 showing positive staining in Cervical carcinoma tissue.

  • Immunofluorescent staining of untreated (C) and Phosphatase- treated (D) A431 cells using ab40815.
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Anti-EGFR (phospho Y1092) antibody [EP774Y] (ab40815) 使用論文

This product has been referenced in:
  • Fu Y  et al. Ductal activation of oncogenic KRAS alone induces sarcomatoid phenotype. Sci Rep 5:13347 (2015). Read more (PubMed: 26289340) »
  • Zhu Z  et al. Phosphorylated AKT1 is associated with poor prognosis in esophageal squamous cell carcinoma. J Exp Clin Cancer Res 34:95 (2015). IHC-P ; Human . Read more (PubMed: 26338103) »

See all 16 Publications for this product

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EGFR is a cell membrane protein, and the signal peptide may be cleaved off after the immature protein enters the endoplasmic reticulum. The 1092 numbering includes the 24 aa signal peptide that is cleaved during ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (stomach cancer tissue)
Loading amount 30 µg
Specification stomach cancer tissue
Treatment 10uM cannabinoid antagonist
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

投稿 Apr 08 2013

Thank you for your enquiry.

ab40815 is a tissue culture supernatant product. It is not a purified antibody. However I can say that, in general, the specific antibody concentration in a product of this type is around 0.05mg/ml.

I hop...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Intestine)
Specification Intestine
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization Yes - 0.1% Tx100 in PBS
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Abcam user community

Verified customer

投稿 Mar 20 2012

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