製品の概要

  • 製品名
    Anti-EGFR antibody [ICR10] (FITC)
    EGFR 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [ICR10] to EGFR (FITC)
  • 標識
    FITC. Ex: 493nm, Em: 528nm
  • アプリケーション
    適用あり: Flow Cytmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    The extracellular domain of human EGFR from head and neck carcinoma.

  • エピトープ
    Binds Epitope B with an affinity of 6.7 x 10-9 M.
  • 特記事項
    This product should be stored undiluted. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/50 - 1/100.

Use 10ul of the suggested working dilution to label 106cells in 100ul.

ab18446-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
      Isoform 2 may act as an antagonist of EGF action.
    • 組織特異性
      Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
    • 関連疾患
      Lung cancer
      Inflammatory skin and bowel disease, neonatal, 2
    • 配列類似性
      Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • 翻訳後修飾
      Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
      Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
      Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
    • 細胞内局在
      Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
    • Information by UniProt
    • 参照データベース
    • 別名
      • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
      • Cell growth inhibiting protein 40 antibody
      • Cell proliferation inducing protein 61 antibody
      • EGF R antibody
      • EGFR antibody
      • EGFR_HUMAN antibody
      • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
      • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
      • Epidermal growth factor receptor antibody
      • erb-b2 receptor tyrosine kinase 1 antibody
      • ERBB antibody
      • ERBB1 antibody
      • Errp antibody
      • HER1 antibody
      • mENA antibody
      • NISBD2 antibody
      • Oncogen ERBB antibody
      • PIG61 antibody
      • Proto-oncogene c-ErbB-1 antibody
      • Receptor tyrosine protein kinase ErbB 1 antibody
      • Receptor tyrosine-protein kinase ErbB-1 antibody
      • SA7 antibody
      • Species antigen 7 antibody
      • Urogastrone antibody
      • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
      • wa2 antibody
      • Wa5 antibody
      see all

    画像

    • Flow Cytometry analysis of A431 cells labeling EGFR with ab11400.

    • Flow Cytometry analysis of A431 cells labeling EGFR with ab11400.

    • ab11400 (1/10 dilution) staining A431 cells by Flow Cytometry. Green - isotype control; Red - ab11400.

    プロトコール

    参考文献

    This product has been referenced in:
    • Kjær I  et al. Cetuximab Resistance in Squamous Carcinomas of the Upper Aerodigestive Tract Is Driven by Receptor Tyrosine Kinase Plasticity: Potential for mAb Mixtures. Mol Cancer Ther 15:1614-26 (2016). Read more (PubMed: 27196767) »
    • Belmont PJ  et al. Resistance to dual blockade of the kinases PI3K and mTOR in KRAS-mutant colorectal cancer models results in combined sensitivity to inhibition of the receptor tyrosine kinase EGFR. Sci Signal 7:ra107 (2014). Read more (PubMed: 25389372) »

    See all 4 Publications for this product

    レビューと Q&A

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab11400.

    To check the status of the order please contact o...

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    Thank you for contacting us.

    The only isotype control we can offer is ab18446; http://www.abcam.com/Rat-IgG2a-kappa-monoclonal-RTK2758-FITC-Isotype-control-ab18446.html

    Please note that the concentration of isotype is not known be...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Balb3T3)
    Specification
    Balb3T3
    Preparation
    Cell harvesting/tissue preparation method: Cultured in DMEM and removed from plate with trypsin/EDTA
    Sample buffer: PBS with 0.1% BSA
    Fixation
    none
    Permeabilization
    No
    Gating Strategy
    forward scatter and side scatter
    Username

    Abcam user community

    Verified customer

    投稿 May 03 2011

    Thank you for your enquiry. The exact epitope sequence recognised by this antibody is not known at the present time. We know that it has been raised against the extracellular domain for the human EGFR and is directed against epitope B (epitopes A-E, ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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