製品の概要

  • 製品名Anti-EGFR antibody [ICR10]
    EGFR 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [ICR10] to EGFR
  • 特異性ICR10 recognises only wild-type Human epidermal growth factor receptor (EGF-R). It does not react with erb-B2.
  • アプリケーション適用あり: Inhibition Assay, Flow Cyt, ELISA, IP, IHC-Fr, ICCmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Extracellular domain of Human EGF-receptor from head and neck carcinoma

  • エピトープICR10 binds to epitope B, and has an affinity of 6.7x10-9 M.
  • ポジティブ・コントロール
    • FACS: A431 cells IHC: Breast Carcinoma tissue WB: HN5 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Inhibition Assay Use at an assay dependent concentration. See ELISA Abreview.
Flow Cyt 1/50 - 1/100.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
      Isoform 2 may act as an antagonist of EGF action.
    • 組織特異性Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
    • 関連疾患Lung cancer
      Inflammatory skin and bowel disease, neonatal, 2
    • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
      Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
      Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
    • 細胞内局在Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
    • Information by UniProt
    • 参照データベース
    • 別名
      • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
      • Cell growth inhibiting protein 40 antibody
      • Cell proliferation inducing protein 61 antibody
      • EGF R antibody
      • EGFR antibody
      • EGFR_HUMAN antibody
      • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
      • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
      • Epidermal growth factor receptor antibody
      • erb-b2 receptor tyrosine kinase 1 antibody
      • ERBB antibody
      • ERBB1 antibody
      • Errp antibody
      • HER1 antibody
      • mENA antibody
      • NISBD2 antibody
      • Oncogen ERBB antibody
      • PIG61 antibody
      • Proto-oncogene c-ErbB-1 antibody
      • Receptor tyrosine protein kinase ErbB 1 antibody
      • Receptor tyrosine-protein kinase ErbB-1 antibody
      • SA7 antibody
      • Species antigen 7 antibody
      • Urogastrone antibody
      • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
      • wa2 antibody
      • Wa5 antibody
      see all

    Anti-EGFR antibody [ICR10] 画像

    • Staining of A431 cells with ab231 visualised with F(ab')2 rabbit anti-rat FITC conjugated secondary.
    • ICC/IF image of ab231 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab231, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

    • ab231 used undiluted in inhibition assay against human recombinant protein EGFR1:Fc to inhibit binding to EGF:Biotin. The primary incubated with sample for 1 hour. The antibody inhibits the binding of biotinylated EGF to coated EGFR:Fc with an IC50 ~ 5 nM.

    • ab231 staining EGFR in human tumor cell line xenograft in mouse by Immunohistochemistry (Frozen sections).
      Tissue was fixed in acetone. Samples incubated with ab231 at a 1/100 dilution for 1 hour at 23°C. The secondary used was an undiluted HRP conjugated goat anti-rat polyclonal.

      See Abreview

    Anti-EGFR antibody [ICR10] (ab231) 使用論文

    This product has been referenced in:
    • Desai TJ  et al. Alveolar progenitor and stem cells in lung development, renewal and cancer. Nature 507:190-4 (2014). Inhibition Assay ; Mouse . Read more (PubMed: 24499815) »
    • Wang J & Yu RK Interaction of ganglioside GD3 with an EGF receptor sustains the self-renewal ability of mouse neural stem cells in vitro. Proc Natl Acad Sci U S A 110:19137-42 (2013). Flow Cyt, ICC/IF ; Mouse . Read more (PubMed: 24198336) »

    See all 8 Publications for this product

    Product Wall

    These four antibodies recognize the extracellular domain of EGFR and are validated for flow cytometry.

    ab231 (clone ICR10)
    ab30 (clone EGFR1)
    ab93051 (clone 31G7)
    ab32198 (clone E234)

    Clones ICR10 and EGFR1 are available d...

    Read More

    Thank you for your inquiry regarding ab231.
    We do not measure the isoelectric point of our antibodies. Most monoclonal antibodies will have an isoelectric point around 6.1, but this is not something that we test.
    Please let me know if you hav...

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    Thank you very much for your reply.

    I've looked at the package'stracking information, and it arrived on September 20 at 9:34 EST. You can track the package on FedEx.com with tracking number: ***. It was signed for by ***- does that help you t...

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    Thank you very much for your reply.

    It does sound like the secondary is reacting with the IgG's in the mouse serum. I'm sending a free of charge vial of ab6115 on the order *** which should arrive tomorrow.

    Please keep me updated ab...

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    Thank you very much for contacting us with your question.

    It is possible that the secondary antibody is causing some background signal, as ab7100 has been pre-adsorbed only against human serum proteins and not mouse serum proteins. Have you d...

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    Thank you for contacting us.

    A common fluorophore used is FITC (green), and we carry ab7100 a rabbit polyclonal secondary antibody to rat iGG - H&L (FITC), pre-adsorbed as ab7100. This has been tested in FC.

    http://www.abcam.co...

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    Thank you for your enquiry and your interest in our products.

    I can confirm that neither of these antibodies have been characterized for inhibition studies. ab24284 is suitable for IHC-P only; whilst ab24293 has been tested for Flow cytometry...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (tumor cell line xenograft in mouse)
    Specification tumor cell line xenograft in mouse
    Fixative Acetone
    Permeabilization No
    Username

    Abcam user community

    Verified customer

    投稿 Feb 24 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application ELISA
    Sample Human Recombinant protein (EGFR1:Fc)
    Specification EGFR1:Fc
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Type Other
    Username

    Ms. Lakshmi Ganesan

    Verified customer

    投稿 Nov 20 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (CB17/SCID BxPC-3 xenograft)
    Specification CB17/SCID BxPC-3 xenograft
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Dr. Jennifer Dembinski

    Verified customer

    投稿 May 14 2009

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"