製品の概要

  • 製品名Anti-EGFR antibody [AT2H8]
    EGFR 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AT2H8] to EGFR
  • アプリケーション適用あり: WB, ELISA, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment purified from E. coli, corresponding to amino acids 424-605 of Human EGFR (NP_005228).

  • ポジティブ・コントロール
    • HaCaT cell extract; HaCaT cells.
  • 特記事項Myeloma: FO cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250. Predicted molecular weight: 134 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF 1/250.
Flow Cyt Use 2-5µg for 106 cells.

ターゲット情報

  • 機能Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.
  • 組織特異性Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
  • 関連疾患Lung cancer
    Inflammatory skin and bowel disease, neonatal, 2
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
    Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
  • 細胞内局在Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
  • Information by UniProt
  • 参照データベース
  • 別名
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • ERBB antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Anti-EGFR antibody [AT2H8] 画像

  • All lanes : Anti-EGFR antibody [AT2H8] (ab124112) at 1/250 dilution

    Lane 1 : HaCaT cell lysate at 10 µg
    Lane 2 : HaCaT cell lysate at 20 µg
    Lane 3 : HaCaT cell lysate at 35 µg

    Secondary
    Goat anti-mouse HRP
    Developed using the ECL technique

    Predicted band size : 134 kDa
    HaCaT extracts were resolved by SDS-PAGE and transferred to PVDF membrane.
  • ab124112 at 1/250 dilution staining EGFR in HaCaT cells by Immunofluorescence. Cells were stained with Hoechst 3342 (Blue) or phalloidin-TRITC for nucleus or actin staining, respectably. ab124112 was c0-stained with alexa 488 (green).
  • Flow cytometry analysis of HeLa cells labelling EGFR with ab124112 at 2-5 µg for 1x106 cells. The secondary antibody used was Alexa Fluor® 488 goat-anti-mouse IgG.

Anti-EGFR antibody [AT2H8] (ab124112) 使用論文

ab124112 has not yet been referenced specifically in any publications.

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