Anti-EGFR Affibody® Molecule FITC (ab81872)

製品の概要

  • 製品名Anti-EGFR Affibody® Molecule FITC
    EGFR affibody® molecules 製品一覧
  • 標識FITC. Ex: 493nm, Em: 528nm
  • 特記事項(標識)The molecule is conjugated at the unique C-terminal cysteine using a maleimide activated fluorescein reagent. Stability: There is no decrease in performance of the fluorescein conjugated Affibody® molecule after 10 repeated freeze and thaw cycles or after storage for 1 week in room temperature.
  • 特異性This product binds to human EGFR. This anti-EGFR Affibody® molecule is a specific affinity ligand selected against the extracellular domain of EGFR. The FITC conjugated Anti-EGFR Affibody® molecule is excellent as a one step reagent for fluorescence studies of EGFR expression on cells as well as for flow cytometry.
  • アプリケーション適用あり: Flow Cyt, ICC/IFmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Other Immunogen Type corresponding to EGFR.

  • ポジティブ・コントロールA431 cells.
  • 特記事項This product is a recombinant protein produced in E.coli.

製品の特性

  • 機能Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.
  • 組織特異性Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
  • 関連疾患Lung cancer
    Inflammatory skin and bowel disease, neonatal, 2
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
    Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
  • 細胞内局在Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
  • Information by UniProt
  • 別名
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog
    • Cell growth inhibiting protein 40
    • Cell proliferation inducing protein 61
    • EGF R
    • EGFR
    • EGFR_HUMAN
    • Epidermal growth factor receptor
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog)
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian)
    • erb-b2 receptor tyrosine kinase 1
    • ERBB
    • ERBB1
    • Errp
    • HER1
    • mENA
    • NISBD2
    • Oncogen ERBB
    • PIG61
    • Proto-oncogene c-ErbB-1
    • Receptor tyrosine protein kinase ErbB 1
    • Receptor tyrosine-protein kinase ErbB-1
    • SA7
    • Species antigen 7
    • Urogastrone
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog
    • wa2
    • Wa5
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab81872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use a concentration of 0.1 - 5 µg/ml.
ICC/IF Use a concentration of 1 - 5 µg/ml.
  • 追加情報Is unsuitable for IHC-P.
  • Anti-EGFR Affibody® Molecule FITC 画像

    • Fluorescence histogram from flow cytometry analysis on EGFR expressing A431 cells. Staining with ab81872 caused an increase in fluorescence intensity as shown by a shift to the right (red line) compared to control (black line).
    • Titration diagram from cytometry analysis of EGFR expressing A431 cells. ab81872 was titrated on EGFR expressing A431 cells. The concentration was plotted against the mean fluorescence intensity (MFI).
    • A431 cells were stained with ab81872 at 2µg/ml by Immunofluorescence. The staining was localized to the membrane of the EGFR-expressing A431 cells. The nuclei were counter stained with DAPI.

    Anti-EGFR Affibody® Molecule FITC (ab81872) 使用論文

    ab81872 has not yet been referenced specifically in any publications.

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    DISCOUNT CODE: *******Expiration date: ****** I am very pleased to hear you would like to accept our offer and test ab81872 inmouse tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit ...

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    Thank you very much for your interest in ab81872. To our knowledge, ab81872 has not been tested in mouse tissue. Therefore, I can offer a discount off a future purchase if you buy ab81872 now, test it in mouse tissue and submit feedback to us in the fo...

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    Thanks for your inquiry. ab81872 does neutralize or block ligand binding. See PubMed reference below:Nucl Med Biol. 2007 Aug;34(6):609-18. From the abstract of that paper: "The cellular binding and processing of an epidermal growth factor...

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    Thank you for contacting us. Please be advised that ab81872 is not a conjugated antibody it is in fact an Affibody molecules. These are a novel class of antibody mimetics with superior characteristics surpassing monoclonal antibodies (mAbs) and ant...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"