製品の概要

  • 製品名Anti-EGF antibody [S-147]
    EGF 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [S-147] to EGF
  • 特異性Reactive with natural and recombinant Human EGF but does not react with other human cytokines tested (IL 1beta, IL 8, MCAF, TGF beta and SAA).
  • アプリケーション適用あり: ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Purified recombinant human EGF. 6kDA, 53 amino acid residues

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • 組織特異性Expressed in kidney, salivary gland, cerebrum and prostate.
  • 関連疾患Hypomagnesemia 4
  • 配列類似性Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • 翻訳後修飾O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta urogastrone antibody
    • EGF antibody
    • EGF_HUMAN antibody
    • Epidermal growth factor antibody
    • HOMG4 antibody
    • OTTHUMP00000219721 antibody
    • OTTHUMP00000219722 antibody
    • Pro epidermal growth factor antibody
    • URG antibody
    • Urogastrone antibody
    see all

Anti-EGF antibody [S-147] 画像

  • ICC/IF image of ab18632 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18632, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-EGF antibody [S-147] (ab18632) 使用論文

ab18632 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"