製品の概要

  • 製品名Anti-EGF antibody [EGF-2]
    EGF 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [EGF-2] to EGF
  • アプリケーション適用あり: WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein (purified) (Human).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • 組織特異性Expressed in kidney, salivary gland, cerebrum and prostate.
  • 関連疾患Hypomagnesemia 4
  • 配列類似性Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • 翻訳後修飾O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta urogastrone antibody
    • EGF antibody
    • EGF_HUMAN antibody
    • Epidermal growth factor antibody
    • HOMG4 antibody
    • OTTHUMP00000219721 antibody
    • OTTHUMP00000219722 antibody
    • Pro epidermal growth factor antibody
    • URG antibody
    • Urogastrone antibody
    see all

Anti-EGF antibody [EGF-2] 画像

  • ICC/IF image of ab8282 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8282, 5µg/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing MCF7 cells stained with ab8282 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8282, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-EGF antibody [EGF-2] (ab8282) 使用論文

ab8282 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your recent telephone enquiry.

I can confirm that recombinant full-size human EGF as an immunogen for ab8282EGF antibody [EGF-2]and intesting. Regrettably, the epitope has not been mapped in this case. As discussed on the telep...

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Merci beaucoup pour votre appel de vendredi passé. Je m'excuse du délais de ma réponse. Veuillez trouver le CoC, le Certificat of Compliance, en document attaché. Je voulais aussi vous confirmer, que nous avons pas de date d'expiration. Nous avo...

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To the best of our knowledge, this antibody has never been tested in rabbit, therefore we can't guarantee results.

We're sorry to hear that you are having problems with this antibody. A 1:3500 dilution is very, very dilute. As you have seen from the datasheet, this anitbody is suitable for use in WB. Please try this antibody at 1:1000, 1:500 and 1:250 dilutions and...

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This is a new antibody and we have not yet tried it in IHC.

I do apologise - there was a mistake on the datasheet which has now been corrected. This antibody is a monoclonal and will therefore recognise a single epitope which has not yet been mapped.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"