製品の概要

  • 製品名Anti-EGF antibody
    EGF 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to EGF
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Highly pure (>98%) recombinant mEGF (mouse Epidermal Growth Factor)

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab9862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

To detect mEGF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mEGF.

WB Use at an assay dependent concentration.

To detect mEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

ターゲット情報

  • 機能EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • 組織特異性Expressed in kidney, salivary gland, cerebrum and prostate.
  • 関連疾患Hypomagnesemia 4
  • 配列類似性Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • 翻訳後修飾O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta urogastrone antibody
    • EGF antibody
    • EGF_HUMAN antibody
    • Epidermal growth factor antibody
    • HOMG4 antibody
    • OTTHUMP00000219721 antibody
    • OTTHUMP00000219722 antibody
    • Pro epidermal growth factor antibody
    • URG antibody
    • Urogastrone antibody
    see all

Anti-EGF antibody (ab9862) 使用論文

This product has been referenced in:
  • Pan Y  et al. The expression of epidermal growth factor (EGF) and its receptor (EGFR) during post-natal testes development in the yak. Reprod Domest Anim 49:970-6 (2014). IHC . Read more (PubMed: 25263304) »

See 1 Publication for this product

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Thank you for your enquiry. This product is a polyclonal or total IgG antibody. It should recognize all subtypes, although we do not perform epitope mapping here.  I'm sorry I couldn't be of more assistance. Please let me know if you have any qu...

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The molecular weight is aprox. 140-150kDa.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"