製品の概要

  • 製品名Anti-EGF antibody
    EGF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EGF
  • アプリケーション適用あり: WB, Neutralising, Indirect ELISA, Sandwich ELISA, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Rat
  • 免疫原

    Highly pure (>98%) recombinant Rat EGF.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 6.2 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralising Use a concentration of 0.1 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of Rat EGF (0.1 ng/ml).
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. In conjunction with compatible secondary reagents, ab77851 allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 21121876
IHC-P Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • 組織特異性Expressed in kidney, salivary gland, cerebrum and prostate.
  • 関連疾患Hypomagnesemia 4
  • 配列類似性Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • 翻訳後修飾O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta urogastrone antibody
    • EGF antibody
    • EGF_HUMAN antibody
    • Epidermal growth factor antibody
    • HOMG4 antibody
    • OTTHUMP00000219721 antibody
    • OTTHUMP00000219722 antibody
    • Pro epidermal growth factor antibody
    • URG antibody
    • Urogastrone antibody
    see all

Anti-EGF antibody (ab77851) 使用論文

This product has been referenced in:
  • Li H  et al. Different expression patterns of growth factors in rat fetuses with spina bifida aperta after in utero mesenchymal stromal cell transplantation. Cytotherapy 16:319-30 (2014). IHC-P ; Rat . Read more (PubMed: 24364908) »
  • Williams CM  et al. Autocrine-controlled formation and function of tissue-like aggregates by primary hepatocytes in micropatterned hydrogel arrays. Tissue Eng Part A 17:1055-68 (2011). ICC/IF ; Rat . Read more (PubMed: 21121876) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"