製品の概要

  • 製品名Anti-Eg5 antibody
    Eg5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Eg5
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1006 and the C terminus (residue 1056) of human Eg5 (NP_004514.2)

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
  • 関連疾患Defects in KIF11 are the cause of microcephaly with or without chorioretinopathy, lymphedema, or mental retardation (MCLMR) [MIM:152950]. An autosomal dominant disorder that involves an overlapping but variable spectrum of central nervous system and ocular developmental anomalies. Microcephaly ranges from mild to severe and is often associated with mild to moderate developmental delay and a characteristic facial phenotype with upslanting palpebral fissures, broad nose with rounded tip, long philtrum with thin upper lip, prominent chin, and prominent ears. Chorioretinopathy is the most common eye abnormality, but retinal folds, microphthalmia, and myopic and hypermetropic astigmatism have also been reported, and some individuals have no overt ocular phenotype. Congenital lymphedema, when present, is typically confined to the dorsa of the feet, and lymphoscintigraphy reveals the absence of radioactive isotope uptake from the webspaces between the toes.
  • 配列類似性Belongs to the kinesin-like protein family. BimC subfamily.
    Contains 1 kinesin-motor domain.
  • 翻訳後修飾Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR.
    A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.
  • 細胞内局在Cytoplasm. Cytoplasm > cytoskeleton > spindle pole.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EG5 antibody
    • HKSP antibody
    • KIF11 antibody
    • KIF11_HUMAN antibody
    • Kinesin family member 11 antibody
    • Kinesin like protein 1 antibody
    • Kinesin-like protein 1 antibody
    • Kinesin-like protein KIF11 antibody
    • Kinesin-like spindle protein HKSP antibody
    • Kinesin-related motor protein Eg5 antibody
    • KNSL1 antibody
    • MCLMR antibody
    • Thyroid receptor-interacting protein 5 antibody
    • TR-interacting protein 5 antibody
    • TRIP-5 antibody
    • TRIP5 antibody
    see all

Anti-Eg5 antibody 画像

  • All lanes : Anti-Eg5 antibody (ab72413) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 120 kDa
    Observed band size : 120 kDa


    Exposure time : 3 seconds
  • Detection of Eg5 by Western Blot of Immunprecipitate.
    ab72413 at 1µg/ml staining Eg5 in HeLa whole cell lysate immunoprecipitated using ab72413 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).
    Detection: Chemiluminescence with exposure time of 1 second.

Anti-Eg5 antibody (ab72413) 使用論文

ab72413 has not yet been referenced specifically in any publications.

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