製品の概要

  • 製品名Anti-EFTUD2 antibody
    EFTUD2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EFTUD2
  • アプリケーション適用あり: IHC-P, ICC/IF, WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Caenorhabditis elegans, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide (Human) corresponding to a region between residues 1 and 50 of EFTUD2. (NP_004238.2)

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
WB 1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 109 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.
  • 関連疾患Defects in EFTUD2 are the cause of mandibulofacial dysostosis with microcephaly (MFDM) [MIM:610536]. A rare syndrome characterized by progressive microcephaly, midface and malar hypoplasia, micrognathia, microtia, dysplastic ears, preauricular skin tags, significant developmental delay, and speech delay. Many patients have major sequelae, including choanal atresia that results in respiratory difficulties, conductive hearing loss, and cleft palate.
  • 配列類似性Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 116 kDa antibody
    • 116 kDa U5 small nuclear ribonucleoprotein component antibody
    • EFTUD2 antibody
    • Elongation factor Tu GTP binding domain containing 2 antibody
    • Elongation factor Tu GTP-binding domain-containing protein 2 antibody
    • hSNU114 antibody
    • MFDGA antibody
    • MFDM antibody
    • SNRNP116 antibody
    • Snrp116 antibody
    • Snu114 antibody
    • SNU114 homolog antibody
    • U5 116KD antibody
    • U5 small nuclear ribonucleoprotein component antibody
    • U5 snRNP specific protein, 116 kD antibody
    • U5 snRNP specific protein, 116 kDa antibody
    • U5 snRNP-specific protein antibody
    • U5-116 kDa antibody
    • U5-116KD antibody
    • U5S1_HUMAN antibody
    see all

Anti-EFTUD2 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling EFTUD2 with ab72456 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-EFTUD2 antibody (ab72456) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 109 kDa
    Observed band size : 117 kDa (why is the actual band size different from the predicted?)
  • HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), with ab72456 used for IP at 3 µg/mg lysate, and at 0.1 µg/ml for subsequent WB.
    Detection: Chemiluminescence with an exposure time of 10 seconds.
  • IHC image of ab72456 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72456, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab110240 staining EFTUD2 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with formaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 5% donkey serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/1000 in PBS) for 1.5 hours at 25°C. A Cy3®-conjugated sheep anti-rabbit polyclonal IgG (1/100) was used as the secondary antibody. DNA was stained with TO-PRO®-3.

    See Abreview

Anti-EFTUD2 antibody (ab72456) 使用論文

ab72456 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization Yes - Triton 0.3%
Blocking step Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jul 02 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"