製品の概要

  • 製品名Anti-EFHD2 antibody
    EFHD2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EFHD2
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (ATDELATKLS RRLQMEGEGG GETPEQPGLN GAAAAAAGAP DEAAEALGSA) of Human EFHD2, NP_077305

  • ポジティブ・コントロール
    • human fetal lung lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab87006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能May regulate B-cell receptor (BCR)-induced immature and primary B-cell apoptosis (By similarity). Plays a role as negative regulator of the canonical NF-kappa-B-activating branch (By similarity). Controls spontaneous apoptosis through the regulation of BCL2L1 abundance.
  • 組織特異性Found in lymphocytes; preferentially expressed in CD8+ cells.
  • 配列類似性Contains 2 EF-hand domains.
  • 細胞内局在Membrane raft. In immature B-cell line WEHI231.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EF hand domain containing protein 2 antibody
    • EF hand domain family, member D2 antibody
    • EF-hand domain-containing protein D2 antibody
    • EFHD 2 antibody
    • EFHD2 antibody
    • EFHD2_HUMAN antibody
    • RP3-467K16.3 antibody
    • Swiprosin 1 antibody
    • Swiprosin-1 antibody
    • SWS1 antibody
    see all

Anti-EFHD2 antibody 画像

  • ab87006 staining EFHD2 in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in 10mM citrate pH6, 0.1% Tween 20. Samples were incubated with primary antibody (diluted in 0.1% TritonX-100 in PBS) for 16 hours at 4°C. An undiluted HRP-conjugated Horse anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

  • Anti-EFHD2 antibody (ab87006) at 1 µg/ml + fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 27 kDa
    Observed band size : 27 kDa
    Gel concentration 12%

Anti-EFHD2 antibody (ab87006) 使用論文

This product has been referenced in:
  • Borger E  et al. The calcium-binding protein EFhd2 modulates synapse formation in vitro and is linked to human dementia. J Neuropathol Exp Neurol 73:1166-82 (2014). IHC . Read more (PubMed: 25383639) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6, 0.1% Tween20
Sample Human Tissue sections (brain tissue)
Specification brain tissue
Permeabilization Yes - 0.1% Tx100
Fixative Formaldehyde
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投稿 May 31 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"