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Our Abpromise guarantee covers the use of ab24368 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 28-30 kDa.|
Immunohistochemistry (Paraffin-embedded sections) analysis of paraffin embedded human tonsil tissue labeling EFHD2 with ab24368 at 2 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
ab24368 staining EFHD2 in Human neuroblastoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS + 5% serum) for 1 hour. DyLight® 594-conjugated Mouse anti-goat IgG polyclonal (7µg/ml) was used as the secondary antibody.
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