製品の概要

  • 製品名
    Anti-EFHA2 antibody
  • 製品の詳細
    Rabbit polyclonal to EFHA2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Cow, Cat, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (

    AAGGGLVGLVCYQLYGDPRAGSPATGRPSKSAATEPEDPPRGRGMLPIPV

    ) of Human EFHA2 (NP_859074)

  • ポジティブ・コントロール
    • 293T cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab89869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

画像

  • Anti-EFHA2 antibody (ab89869) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 61 kDa

プロトコール

参考文献

ab89869 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (4-12% BIS-TRIS)
Sample
Human Cell lysate - whole cell (fibroblasts)
Specification
fibroblasts
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Feb 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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