Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling EEF2K with ab85721 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - EEF2K antibody (ab85721)
All lanes : Anti-EEF2K antibody (ab85721) at 0.1 µg/ml
Lane 1 : HeLa cells lysate at 50 µg Lane 2 : HeLa cells lysate at 15 µg Lane 3 : HeLa cells lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 95 kDa Observed band size: 95 kDa Additional bands at: 110 kDa, 350 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Immunoprecipitation - EEF2K antibody (ab85721)
Detection of Human EEF2K by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab85721 at 3 ug/mg lysate. Subsequent Western blot detection was performed using 1 ug/ml ab85721.
Detection: Chemiluminescence with exposure times of 30