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RabMAb

Anti-EEF2 (phospho T56 + T58) 抗体 [EP833Y] (ab82981)

製品の概要

  • 製品名
    Anti-EEF2 (phospho T56 + T58) antibody [EP833Y]
    EEF2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP833Y] to EEF2 (phospho T56 + T58)
  • 特異性
    The antibody only detects eEF2 phosphorylated on Tyreonine 56/58.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Thr 56/58 of human EEF2

  • ポジティブ・コントロール
    • 293 cell lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EP833Y
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 95 kDa.

ターゲット情報

  • 機能
    Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
  • 配列類似性
    Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
  • 翻訳後修飾
    Phosphorylation by EF-2 kinase completely inactivates EF-2.
    Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
    ISGylated.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EEF 2 antibody
    • Eef2 antibody
    • EF-2 antibody
    • EF2 antibody
    • EF2_HUMAN antibody
    • Elongation factor 2 antibody
    • Eukaryotic translation elongation factor 2 antibody
    • Polypeptidyl tRNA translocase antibody
    • SCA26 antibody
    see all

画像

  • All lanes : Anti-EEF2 (phospho T56 + T58) antibody [EP833Y] (ab82981) at 1/2000 dilution

    Lane 1 : Untreated 293 cell lysate
    Lane 2 : Serum starved 293 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 95 kDa
    Observed band size : 95 kDa

プロトコール

参考文献

This product has been referenced in:
  • Tavares CD  et al. The molecular mechanism of eukaryotic elongation factor 2 kinase activation. J Biol Chem 289:23901-16 (2014). Human . Read more (PubMed: 25012662) »
  • Hamdi MM & Mutungi G Dihydrotestosterone stimulates amino acid uptake and the expression of LAT2 in mouse skeletal muscle fibres through an ERK1/2-dependent mechanism. J Physiol 589:3623-40 (2011). WB ; Mouse . Read more (PubMed: 21606113) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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