製品の概要

  • 製品名Anti-EEF2 (phospho T56) antibody
    EEF2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EEF2 (phospho T56)
  • 特異性ab53114 detects endogenous levels of EEF2 only when phosphorylated at threonine 56.
  • アプリケーション適用あり: ICC/IF, WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human EEF2.

  • ポジティブ・コントロール
    • Human breast carcinoma tissue and extracts from NIH 3T3 cells treated with serum.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab53114 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab53114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 24130777
WB 1/300 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
  • 配列類似性Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
  • 翻訳後修飾Phosphorylation by EF-2 kinase completely inactivates EF-2.
    Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
    ISGylated.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EEF 2 antibody
    • Eef2 antibody
    • EF-2 antibody
    • EF2 antibody
    • EF2_HUMAN antibody
    • Elongation factor 2 antibody
    • Eukaryotic translation elongation factor 2 antibody
    • Polypeptidyl tRNA translocase antibody
    • SCA26 antibody
    see all

Anti-EEF2 (phospho T56) antibody 画像

  • All lanes : Anti-EEF2 (phospho T56) antibody (ab53114) at 1/300 dilution

    Lane 1 : Extract from NIH 3T3 cells treated with serum (10%, 30mins) with Immunising peptide
    Lane 2 : Extract from NIH 3T3 cells treated with serum (10%, 30mins)


    Predicted band size : 95 kDa
    Observed band size : 95 kDa
  • ab53114 at 1/50 dilution staining EEF2 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.ab53114 at 1/50 dilution staining EEF2 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

Anti-EEF2 (phospho T56) antibody (ab53114) 使用論文

This product has been referenced in:
  • Grandjean M  et al. Hypoxia Integration in the Serological Proteome Analysis Unmasks Tumor Antigens and Fosters the Identification of Anti-Phospho-eEF2 Antibodies as Potential Cancer Biomarkers. PLoS One 8:e76508 (2013). WB, ICC/IF ; Mouse, Human . Read more (PubMed: 24130777) »
  • Collodoro M  et al. Identification and quantification of concentration-dependent biomarkers in MCF-7/BOS cells exposed to 17ß-estradiol by 2-D DIGE and label-free proteomics. J Proteomics 75:4555-69 (2012). WB ; Human . Read more (PubMed: 22580036) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab53114.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"