Anti-EEF2/Elongation factor 2 (phospho T56) 抗体 (ab53114)
Key features and details
- Rabbit polyclonal to EEF2/Elongation factor 2 (phospho T56)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-EEF2/Elongation factor 2 (phospho T56) antibody
EEF2/Elongation factor 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EEF2/Elongation factor 2 (phospho T56) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human EEF2/Elongation factor 2 aa 1-100 (phospho T56).
Database link: P13639 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab53114 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53114の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/300 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
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IHC-P |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/300 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa). |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. -
配列類似性
Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily. -
翻訳後修飾
Phosphorylation by EF-2 kinase completely inactivates EF-2.
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
ISGylated. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 1938 Human
- Entrez Gene: 13629 Mouse
- Omim: 130610 Human
- SwissProt: P13639 Human
- SwissProt: P58252 Mouse
- Unigene: 515070 Human
- Unigene: 326799 Mouse
- Unigene: 379638 Mouse
see all -
別名
- EEF 2 antibody
- Eef2 antibody
- EF-2 antibody
see all
画像
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All lanes : Anti-EEF2/Elongation factor 2 (phospho T56) antibody (ab53114) at 1/300 dilution
Lane 1 : Extract from NIH 3T3 cells
treated with serum (10%, 30mins) with Immunising peptide
Lane 2 : Extract from NIH 3T3 cells
treated with serum (10%, 30mins)
Predicted band size: 95 kDa
Observed band size: 95 kDa -
ab53114 at 1/50 dilution staining EEF2/Elongation factor 2 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab53114 は 4 報の論文で使用されています。
- Ma YF et al. Phosphorylation of AKT serine/threonine kinase and abundance of milk protein synthesis gene networks in mammary tissue in response to supply of methionine in periparturient Holstein cows. J Dairy Sci 102:4264-4274 (2019). PubMed: 30879806
- Delaidelli A et al. MYCN amplified neuroblastoma requires the mRNA translation regulator eEF2 kinase to adapt to nutrient deprivation. Cell Death Differ 24:1564-1576 (2017). PubMed: 28574509
- Grandjean M et al. Hypoxia Integration in the Serological Proteome Analysis Unmasks Tumor Antigens and Fosters the Identification of Anti-Phospho-eEF2 Antibodies as Potential Cancer Biomarkers. PLoS One 8:e76508 (2013). WB, ICC/IF ; Mouse, Human . PubMed: 24130777
- Collodoro M et al. Identification and quantification of concentration-dependent biomarkers in MCF-7/BOS cells exposed to 17ß-estradiol by 2-D DIGE and label-free proteomics. J Proteomics 75:4555-69 (2012). WB ; Human . PubMed: 22580036