製品の概要

  • 製品名Anti-EEF2 antibody
    EEF2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EEF2
  • 特異性ab53139 detects endogenous levels of total eEF2 protein.
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthesized non phosphopeptide derived from human eEF2 around the phosphorylation site of threonine 56 (RFTDT)

  • ポジティブ・コントロール
    • WB: extracts from NIH/3T3 cells IHC-P: Human breast carcinoma tissue

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab53139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/300 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

ターゲット情報

  • 機能Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
  • 配列類似性Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
  • 翻訳後修飾Phosphorylation by EF-2 kinase completely inactivates EF-2.
    Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
    ISGylated.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EEF 2 antibody
    • Eef2 antibody
    • EF-2 antibody
    • EF2 antibody
    • EF2_HUMAN antibody
    • Elongation factor 2 antibody
    • Eukaryotic translation elongation factor 2 antibody
    • Polypeptidyl tRNA translocase antibody
    • SCA26 antibody
    see all

Anti-EEF2 antibody 画像

  • All lanes : Anti-EEF2 antibody (ab53139) at 1/300 dilution

    Lane 1 : NIH/3T3 cells treated with serum (10%, 30mins), no immunising peptide
    Lane 2 : NIH/3T3 cells treated with serum (10%, 30mins), with immunising peptide


    Predicted band size : 95 kDa
    Observed band size : 95 kDa
  • ab53139, at a 1/50 dilution, staining eEF2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry. Left image: no peptide. Right image: immunising peptide present.

Anti-EEF2 antibody (ab53139) 使用論文

This product has been referenced in:
  • Collodoro M  et al. Identification and quantification of concentration-dependent biomarkers in MCF-7/BOS cells exposed to 17ß-estradiol by 2-D DIGE and label-free proteomics. J Proteomics 75:4555-69 (2012). WB ; Human . Read more (PubMed: 22580036) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab53139.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"