保存方法 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファー Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
精製度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
Use a concentration of 1 µg/ml.
機能 EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.
配列類似性 Belongs to the EF-1-beta/EF-1-delta family. Contains 1 GST C-terminal domain.
翻訳後修飾 Phosphorylation affects the GDP/GTP exchange rate.
Information by UniProt
Anti-eEF1B2 antibody 画像
Western blot - eEF1B2 antibody (ab37957)
All lanes : Anti-eEF1B2 antibody (ab37957) at 1/250 dilution Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat whole cell lysate ( ab7899) Lane 3 : A431 whole cell lysate ( ab7909) Lane 4 : HEK293 whole cell lysate ( ab7902) Lane 5 : HepG2 whole cell lysate ( ab7900) Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 7 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution Performed under reducing conditions. Predicted band size : 25 kDa Observed band size : 30 kDa ( why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - eEF1B2 antibody (ab37957)
ICC/IF image of ab37957 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37957, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Anti-eEF1B2 antibody (ab37957) 使用論文
has not yet been referenced specifically in any publications.
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