製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to EEF1A2
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human, Zebrafish
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Pig
  • 免疫原

    Synthetic peptide: VIDCHTAHIA CKFAELKEKI DRRSGKKLED NPKSLKSGDA AIVEMVPGKP, corresponding to a region within internal sequence amino acids 360 - 409 of Human EEF1A2 (NP_001949)

  • ポジティブ・コントロール
    • Fetal brain lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/12500.

ターゲット情報

画像

  • Anti-EEF1A2 antibody (ab82912) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • All lanes : Anti-EEF1A2 antibody (ab82912) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

プロトコール

参考文献

ab82912 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab82912.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録