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Synthetic peptide: VIDCHTAHIA CKFAELKEKI DRRSGKKLED NPKSLKSGDA AIVEMVPGKP, corresponding to a region within internal sequence amino acids 360 - 409 of Human EEF1A2 (NP_001949)
Our Abpromise guarantee covers the use of ab82912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/12500.
ab82912 has not yet been referenced specifically in any publications.