製品の概要

  • 製品名Anti-eEF1A1 antibody
    eEF1A1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to eEF1A1
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Rat
    交差が予測される動物種: Mouse, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 131-180 (GQTREHALLA YTLGVKQLIV GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK) of Rat eEF1A1 (XP_002725693.1).

  • ポジティブ・コントロール
    • Rat liver lysate IF/ICC: HeLa cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab118703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
  • 組織特異性Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle.
  • 配列類似性Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
  • 翻訳後修飾ISGylated.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
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    see all

Anti-eEF1A1 antibody 画像

  • ab118703 stained HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab118703 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-eEF1A1 antibody (ab118703) at 1 µg/ml + Rat liver lysate at 10 µg

    Predicted band size : 50 kDa
    Gel concentration: 12%

Anti-eEF1A1 antibody (ab118703) 使用論文

ab118703 has not yet been referenced specifically in any publications.

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