製品の概要

  • 製品名Anti-EEA1 antibody [mAbcam18175]
    EEA1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [mAbcam18175] to EEA1
  • アプリケーション適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Chicken, Cow, Dog, Chimpanzee, Zebrafish
  • 免疫原

    Synthetic peptide derived from within residues 1350 to the C-terminus of Human EEA1.

    (Peptide available as ab14946.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HeLa cells in Flow Cytometry.
  • 特記事項

    Alternative versions available:

    Anti-EEA1 antibody (FITC) [mAbcam18175] (ab150269)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 162 kDa).Can be blocked with Human EEA1 peptide (ab14946).
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
  • 配列類似性Contains 1 C2H2-type zinc finger.
    Contains 1 FYVE-type zinc finger.
  • ドメインThe FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • 細胞内局在Cytoplasm. Early endosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Early endosome antigen 1 antibody
    • Early endosome antigen 1, 162kD antibody
    • Early endosome associated protein antibody
    • EEA 1 antibody
    • EEA1 antibody
    • EEA1_HUMAN antibody
    • Endosome associated protein p162 antibody
    • Endosome-associated protein p162 antibody
    • MST105 antibody
    • MSTP105 antibody
    • ZFYVE2 antibody
    • Zinc finger FYVE domain containing protein 2 antibody
    • Zinc finger FYVE domain-containing protein 2 antibody
    see all

Anti-EEA1 antibody [mAbcam18175] 画像

  • All lanes : Anti-EEA1 antibody [mAbcam18175] (ab18175) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : A431 whole cell lysate (ab7909)
    Lane 3 : HEK293 whole cell lysate (ab7902)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 162 kDa

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab18175 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18175, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-EEA1 antibody [mAbcam18175] (ab18175) 使用論文

This product has been referenced in:
  • Liu J  et al. Rhbdd3 controls autoimmunity by suppressing the production of IL-6 by dendritic cells via K27-linked ubiquitination of the regulator NEMO. Nat Immunol 15:612-22 (2014). Read more (PubMed: 24859449) »
  • Zhou H  et al. Analysis of the subcellular phosphoproteome using a novel phosphoproteomic reactor. J Proteome Res 9:1279-88 (2010). Read more (PubMed: 20067319) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Pancreatic acinar explants)
Gel Running Conditions Reduced Denaturing (12.5%)
Loading amount 10 µg
Specification Pancreatic acinar explants
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 19°C
Username

Dr. Clara Lubeseder-Martellato

Verified customer

投稿 Jun 16 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application IHC - Wholemount
Sample Zebrafish Embryo (whole-mount of early gastrula)
Specification whole-mount of early gastrula
Username

Mr. Norman Gerstner

Verified customer

投稿 Mar 26 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry free floating
Sample Mouse Tissue sections (Brain)
Specification Brain
Username

Dr. Sophie Pezet

Verified customer

投稿 Jul 29 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (RAW 264.7 macrophages cell line)
Loading amount 15 µg
Specification RAW 264.7 macrophages cell line
Gel Running Conditions Reduced Denaturing (4-12% bis-tris)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jul 31 2008

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (RPE-1)
Specification RPE-1
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

投稿 Sep 04 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"