製品の概要

  • 製品名Anti-EEA1 antibody
    EEA1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EEA1
  • アプリケーション適用あり: WBmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Platypus (Ornithorhynchus anatinus), Elephant
  • 免疫原

    Synthetic peptide corresponding to a region within the C terminal amino acids 1361-1411 (CGKGFSVTVR RHHCRQCGNI FCAECSAKNA LTPSSKKPVR VCDACFNDLQ G) of Human EEA1, NP_003557.2

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells, 293T cells and NIH3T3 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 162 kDa).
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
    • 配列類似性Contains 1 C2H2-type zinc finger.
      Contains 1 FYVE-type zinc finger.
    • ドメインThe FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
    • 細胞内局在Cytoplasm. Early endosome membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Early endosome antigen 1 antibody
      • Early endosome antigen 1, 162kD antibody
      • Early endosome associated protein antibody
      • EEA 1 antibody
      • EEA1 antibody
      • EEA1_HUMAN antibody
      • Endosome associated protein p162 antibody
      • Endosome-associated protein p162 antibody
      • MST105 antibody
      • MSTP105 antibody
      • ZFYVE2 antibody
      • Zinc finger FYVE domain containing protein 2 antibody
      • Zinc finger FYVE domain-containing protein 2 antibody
      see all

    Anti-EEA1 antibody 画像

    • All lanes : Anti-EEA1 antibody (ab86566) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg
      Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg


      Predicted band size : 162 kDa
      Observed band size : 200 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

    Anti-EEA1 antibody (ab86566) 使用論文

    ab86566 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab86566.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"