製品の概要

  • 製品名Anti-EEA1 antibody
    EEA1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EEA1
  • アプリケーション適用あり: WB, ICC, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    GSQGSDLDSSATPINTVDVNNESSSEG

    conjugated to KLH by a C-terminal Cysteine residue linker, corresponding to amino acids 24 - 40 of Human EEA1

  • ポジティブ・コントロール
    • whole extract of mouse NIH-3T3 cells; human HeLa and rat NRK cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC
ICC/IF
  • 追加情報ICC/IF: Use at a concentration of 5 - 10 µg/ml.
    WB: Use at a concentration of 0.4 - 0.8 µg/ml. Detects a band of approximately 160 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
    • 配列類似性Contains 1 C2H2-type zinc finger.
      Contains 1 FYVE-type zinc finger.
    • ドメインThe FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
    • 細胞内局在Cytoplasm. Early endosome membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Early endosome antigen 1 antibody
      • Early endosome antigen 1, 162kD antibody
      • Early endosome associated protein antibody
      • EEA 1 antibody
      • EEA1 antibody
      • EEA1_HUMAN antibody
      • Endosome associated protein p162 antibody
      • Endosome-associated protein p162 antibody
      • MST105 antibody
      • MSTP105 antibody
      • ZFYVE2 antibody
      • Zinc finger FYVE domain containing protein 2 antibody
      • Zinc finger FYVE domain-containing protein 2 antibody
      see all

    Anti-EEA1 antibody 画像

    • HeLa cells fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1 µg/mL ab50313."
    • ab50313 staining EEA1 in mouse primary astrocytes by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.05% saponin and blocking with 10% serum was performed for 20 minutes at 250C. Samples were incubated with primary antibody (1/150: in 10% goat serum / PBS) for 1 hour at 25°C. An Abcam`s ab6939, Cy3®-conjugated goat polyclonal to rabbit IgG (H&L) was used as secondary antibody at 1/200 dilution.

      See Abreview

    Anti-EEA1 antibody (ab50313) 使用論文

    This product has been referenced in:
    • Schorch B  et al. LRP1 is a receptor for Clostridium perfringens TpeL toxin indicating a two-receptor model of clostridial glycosylating toxins. Proc Natl Acad Sci U S A 111:6431-6 (2014). ICC/IF ; Mouse . Read more (PubMed: 24737893) »
    • Glauser DL  et al. Virion endocytosis is a major target for murid herpesvirus-4 neutralization. J Gen Virol 93:1316-27 (2012). Read more (PubMed: 22377583) »

    See all 6 Publications for this product

    Product Wall

    Thank you very much for your responses.
    I'm sending a free of charge vial of ab95195 on the order ***, which should arrive tomorrow. Please keep me updated about the results using this antibody.
    For the replacement of ab50313, I certainly und...

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    Thank you very much for your reply and for sending all of this information.
    I will be happy to send the replacement ab95195, but I will need the original order number or PO number that ab2074 was received on. Would you be able to provide this info...

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    Thanks for the update. We certainly appreciate your lab's troubleshooting efforts. Please feel free to reply after your trip.

    We have previously been in contact regarding ab2074, which was not providing satisfactory results. We had offered a free of charge replacement with a different antibody, but we were waiting to hear from you with your selection. If you have dete...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Mouse Cultured Cells (primary astrocyte)
    Specification primary astrocyte
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.05% saponin
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Sep 08 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Lung carcinoma cell line)
    Specification Lung carcinoma cell line
    Fixative Paraformaldehyde
    Permeabilization Yes - Triton X-100
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    投稿 Aug 17 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"